Sprague-Dawley계 정상 흰쥐에서 위점막 알코올 탈수소효소 활성에 대한 성별의 영향

성기철; 강주섭; 이창호; 고현철; 신인철; 강석한; 전용철; 엄애선

추천

검색

자료유형: 학술저널

저자정보: 성기철 (한양대학교 의과대학 약리학교실 및 의과학연구소) 강주섭 (한양대학교 의과대학 약리학교실 및 의과학연구소) 이창호 (한양대학교 의과대학 약리학교실 및 의과학연구소) 고현철 (한양대학교 의과대학 약리학교실 및 의과학연구소) 신인철 (한양대학교 의과대학 약리학교실 및 의과학연구소) 강석한 (한양대학교 의과대학 생리학교실) 전용철 (한양대학교 의과대학 내과학교실) 엄애선 (한양대학교 의과대학 생활과학대학 식품영양학과)

저널정보: 한국응용약물학회 The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 제8권 제1호

발행연도: 2000.1

수록면: 38 - 43 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Several studies have shown that the stomach has sufficient alcohol dehydrogenase (ADH) activity to metabolize some amount of orally administered alcohol and the sex-related differences in the first-pass metabolism of alcohol might be associated with differences in the activity of gastric ADH(GADH). The aim of this study was to asses the sex-related differences in GADH in 48 male and 48 female Sprague-Dawley rats aged 1, 4, 10, 15, 20, and 30 weeks which each aged group had same sex ratio. The GADH activity was determined spectrophotometrically at 37$^{\circ}C$. The formation of NADH was monitored at 340nm for 10 minutes in the 1 ml of reaction mixture (0.5 M of Tris-HCl, pH 7.2 + 1.5 M of ethanol + 2.8 mM of NAD + 30 $\mu$l gastric mucosal supernatant). The GADH activity (nM of NADH/min/mg of cytosolic protein) was calculated using molecular extinction coefficient of 6.22 $\textrm{cm}^2$/$\mu$M for NADH. The GADH activities were 2.94$\pm$0.82 (n=48) in female rats and 3.34$\pm$2.17 (n=48) in male rats and had not significant difference between sex. However, the GADH activities were significantly (p<0.01) higher in female (1.91$\pm$0.59 and 3.30$\pm$0.49) than in male (0.68$\pm$0.43 and 1.92$\pm$0.81) of 1 and 4 weeks rats. However, it was significantly (p<0.05) higher in male (6.48$\pm$1.81, 3.65$\pm$1.04 and 5.13$\pm$1.30) than in female (4.23$\pm$1.23, 2.18$\pm$0.77 and 2.56$\pm$0.93) of 10, 20 and 30 weeks rats, respectively. Therefore, the results suggested that sex-related differences of the GADH activities in same aged rats were existed by age.

#Gastric alcohol dehydrogenase #GADH) #FPM #First-pass metabolism) #Sex-related differences #ADH activity

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!