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논문 기본 정보

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학술저널
저자정보
Lee, Hee-Ryung (Bio-Safety Research Institute, Chonbuk National university) Lim, Chae-Woong (Bio-Safety Research Institute, Chonbuk National university) Mo, In-Pil (National Veterinary Research Institute) Moon, Hyung-Bae (Department of Pathology, School of Medicine, Wankwang University) Kim, Ki-Suck (National Veterinary Research Institute) Rim, Byung-Moo (Bio-Safety Research Institute, Chonbuk National university)
저널정보
한국수의병리학회 한국수의병리학회지 한국수의병리학회지 제2권 제1호
발행연도
1998.1
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1 - 8 (8page)

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Chickens at 3-weeks of age were inoculated with a highly virulent strain (SH/92) of Infectious Bursal Disease Virus(IBDV) through ocular and cloacal routes. The infected chickens were killed at 6, 12, 24, 48, 72, and 96 hrs post inoculation (PI) and Bursa of Fabricius(BF) were collected. The sizes of bursal follicules in infected chickens decreased at 48 to 96 hrs PI. Histologically the cellular changes were first evident at 12 hrs PI and characterized by condensation of nuclear chromatin of bursal lymphocytes indicating apoptosis. By 24 hrs PI apoptotic lymphocytes dramatically increased. In addition infiltration of heterophils were also seen in the follicles and in the interfollicular connective tissues. At 48 hrs PI, cystic cavities were observed in the follicles. As the infection advanced the bursal follicles showed atrophy accompanied by disappearance of heterophils and reduction in number of lymphocytes in the cystic cavities which was replaced by proteineous materials. The nuclei of most affected lymphocyte stained positively with the in situ end labeling for apoptosis. Electron microscopy showed viral particles with crystalline array in the lymphocytes of BF infected with IBOV. These results indicated that SH/92 IBDV infection in chickens caused increased apoptosis in the BF.

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