Use of a Xanthine-Xanthine Oxidase System on In Vitro Maturation and Fertilization in Pig

Sa, S.J.; Park, C.K.; Cheong, H.T.; Yang, B.K.; Kim, C.I.

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자료유형: 학술대회자료

저자정보: Sa, S.J. (College of Animal Resource Science, Kangwon University) Park, C.K. (College of Animal Resource Science, Kangwon University) Cheong, H.T. (College of Animal Resource Science, Kangwon University) Yang, B.K. (College of Animal Resource Science, Kangwon University) Kim, C.I. (College of Animal Resource Science, Kangwon University)

저널정보: 한국동물번식학회 한국동물번식학회 학술대회 한국동물번식학회 2001년도 춘계학술발표대회

발행연도: 2001.1

수록면: 13 - 13 (1page)

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This study was undertaken to evaluate the effects of catalase using xanthine (X) - xanthine oxidase (XO) system on in vitro maturation and fertilization in pig. When follicular oocytes were cultured in maturation medium with X and/or XO, the maturation rates were not significantly different between in medium with and without catalase despite of different culture periods. However, significantly (P<0.05) higher maturation rates were obrained in culture with X-XO system. The rates of degenerated oocytes were increased with culture periods prolonged, and were significantly (P<0.05) higher in medium without than with catalase at 120 h of culture. On the other hand, the parthenogenetic oocytes were observed with high proportions at 72 h of culture, hut were not different in medium with and without catalase at various times of culture. In another experiment, the frozen-thawed boar spermatozoa treated with X-XO system for in vitro fertilization. The penetration rates were higher in medium with that than without catalase during the in vitro fertilization with, none (P<0.05), XO and X＋XO. On the other hand, when sperm were treated with none, X, XO and X＋XO, lipid peroxidation were higher in medium without that than with catalase. However, the changes in sperm penetration and lipid peroxidation showed opposite patterns. The sperm suspensions were also treated with X and/or XO for assay of sulfhydryl (-SH) group content. Under the above all conditions, sperm-SH group were higher detected In medium with that than without catalase. The activity of sperm binding to zona pellucida was also evaluated through binding to salt-stored porcine oocytes. In control group, sperm binding to zona pellucida were higher than in medium with X, XO and X＋XO groups. No significant differences, however, were observed between medium with and without catalase. In conclusion, the exposure of follicular oocytes and spermatozoa to X-XO system may be caused stimulating in vitro maturation and fertilization in pig. This work was supported by grant No. 2000-1-22200-001-3 from the Basic Research Program of the Korea Science & Engineering Foundation.

#catalase #IVM #IVF #pig #xanthine-xanthine oxidase

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