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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 60 - 60 (1page)
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GSH-dependent glutaredoxinl (Grxl) was characterized in Dictyostelium discoideum. After starvation, the mRNA levels of grx1 gene increased during aggregation, thereafter decreased up to tip formation and increased again during culmination. To investigate the function of Grxl, the protein was overexpressed in D. discoideum using actinl5 promoter, The phenotype analysis on Grxl-overexpressed cells showed the maintenance of slug stage for a long period and delayed culmination under dark condition. To corroborate these phenotype by the enzyme, the two mutant forms of Grxl (C21S and C24S) were overexpressed in D. discoideum. The phenotype of two mutant cells represented no slug formation and the early culmination on dark condition. These results indicate that Grxl might regulate the transition from slug to culminant in darkness.
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