Effect of Sugar Combination in Tris-buffer on the Viability of Post-thaw Spermatozoa in Canine

Yu, D.J.; Jeong, S.R.; Oh, I.S.; Bae, I.H.; Cho, S.G.; Kong, I.K.

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자료유형: 학술대회자료

저자정보: Yu, D.J. (Department of Animal Science and Technology, Sunchon National University) Jeong, S.R. (Department of Animal Science and Technology, Sunchon National University) Oh, I.S. (Department of Animal Science and Technology, Sunchon National University) Bae, I.H. (Department of Animal Science and Technology, Sunchon National University) Cho, S.G. (Department of Animal Science and Technology, Sunchon National University) Kong, I.K. (Department of Animal Science and Technology, Sunchon National University)

저널정보: 한국동물번식학회 한국수정란이식학회 심포지움 한국수정란이식학회 2002년도 국제심포지엄

발행연도: 2002.1

수록면: 90 - 90 (1page)

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The purpose of this study was to investigate the effect of kind and combination of sugars on the viability and acrosome damage of post-thaw spermatozoa in canine. The extender used was Tris-citric acid extender (Tris-buffer) supplemented with 20% Egg-yolk, 8% glycerol, 1% Equex STM paste, and 70 mM sugars such as monosaccharide (fructose and xylose) and disaccharide(trehalose). To evaluate of sugar combination, the sugars supplemented in Tris-buffer were combined such as single (fructose, xylose, trehalose), two combinations (Fruc+Tre, Fruc+xyl, Tre+xyl) and three combinations (Fruc+Tre+Xyl). The concentration of sperm collected were adjusted of 50${\times}$10$\^$6/ per straw for freezing. The frozen spermatozoa were thawed at 37$^{\circ}C$ for 1 min and then analysis for CASA program in Livestock Improvement Main Center, NACF. The motility of post-thaw spermatozoa in Fruc+Tre was higher than those in fructose, trehalose, xylose, Fru+xyl, Tre+xyl and Fru+Tre+Xyl (79％ vs. 63, 66, 70, 71, 74 and 75%). The progressive motility after CASA analysis in Fuc+Tre group was also higher than those in fructose, trehalose, xylose, Fru+xyl, Tre+xyl and Fru+Tre+Xyl (67% vs. 53, 57, 60, 61, 62 and 64%). The acrosome damage of post-thaw spermatozoa stained was not significantly different among treatment groups such as fructose, trehalose, xylose, Fru+Tre, Fru+xyl, Tre+xyl and Freu+tre+xyl (17.7, 18.3, 28.0, 17.0, 19.7, 20.0 and 19.0%). The results indicated that the motility and progressive motility of post-thaw spermatozoa in Fru+Tre group was better, and acrosome normality was not different among all groups. The use of Tris-buffer supplemented with Fru+Tre as sugar for frezing of canine spermatosoa could be better and apply to semen banking and artificial insemination.

#canine #spermatozoa #frozen #sugar #CASA

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