지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.6
- 수록면
- 183 - 191 (9page)
이용수
초록· 키워드
오류제보하기
Emission of greenhouse gases into the atmosphere by human activities leads to global warming. To reduce the level of CO₂ the bio-catalytic properties of microbial carbonic anhydrase (CA) can be exploited. The present study aimed to isolate CA producing bacteria from cow saliva. After thorough screening for CA activity in the bacterial cultures, ten isolates were selected. Out of ten bacterial isolates, T5 isolate showed the highest CA activity (83.92 U/mL) and the isolate was identified as Corynebacterium flavescens using 16s rRNA analysis. Various production parameters for the optimum production of CA were optimized. During the optimization, incubation temperature 40℃, agitation speed 120 rpm, and inoculum volume 4%v/v was found to be optimum for CA production. The optimum reaction pH, reaction time and temperature were 7, 10 min and 35℃, respectively. The crude enzyme was tested for the conversion of CO₂ into the calcium carbonate (CaCO₃) under controlled conditions. The CO₂ conversion efficacy of crude CA was observed to be ~45 ㎎ CaCO₃/mg protein. The synthesized CaCO₃ was analyzed using scanning electron microscopy and X-ray diffraction techniques for particle size, morphology and elemental structure. Calcite precipitation by bacterial CA makes it a potential candidate to be effectively employed in biomimetic CO₂ sequestration.
목차
ABSTRACT
1. Introduction
2. Methods
3. Results and Discussion
4. Conclusions
References
참고문헌 (44)
참고문헌 신청
Sharma T, Sharma S, Kamyab H, et al. Energizing the CO2 utilization by chemo-enzymatic approaches and potentiality of carbonic anhydrases: A review. J. Clean. Prod. 2020;247:1-12.
Zhang W, Liu H, Sun C, et al. Capturing CO2 from ambient air using a polyethyleneimine–silica adsorbent in fluidized beds. Chem. Eng. Sci. 2014;116:306-316.
Mulenga D, Siziya S. Indoor Air Pollution Related Respiratory Ill Health, a Sequel of Biomass Use. SciMed. J. 2019;30-37.
DiMario RJ, Machingura MC, Waldrop GL et al. The many types of carbonic anhydrases in photosynthetic organisms. Plant Sci. 2018;268:11–17.
Kupriyanova EV, Sinetova MA, Cho SM, et al. CO2-concentrating mechanism in cyanobacterial photosynthesis: organization, physiological role, and evolutionary origin. Photosynth Res. 2013;117(1-3):133-46.
Zhang W, Liu H, Sun C, et al. Capturing CO2 from ambient air using a polyethyleneimine–silica adsorbent in fluidized beds. Chem. Eng. Sci. 2014;116:306-316.
Mulenga D, Siziya S. Indoor Air Pollution Related Respiratory Ill Health, a Sequel of Biomass Use. SciMed. J. 2019;30-37.
DiMario RJ, Machingura MC, Waldrop GL et al. The many types of carbonic anhydrases in photosynthetic organisms. Plant Sci. 2018;268:11–17.
Kupriyanova EV, Sinetova MA, Cho SM, et al. CO2-concentrating mechanism in cyanobacterial photosynthesis: organization, physiological role, and evolutionary origin. Photosynth Res. 2013;117(1-3):133-46.
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