지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Biogenic polyamines have been shown previously to reduce oxidative stress in bacteria exposed to antibiotics. The polyamine protective effect could be due to either the scavenging of ROS or interfering with the binding of antibiotics to target macromolecules. To assess the contribution of each mechanism, a comparative study of the effect of polyamines (putrescine, cadaverine, spermidine, and 1,3-diaminopropane) and wellknown antioxidants (Trolox, thiourea) on the intensity of reactive oxygen species production in vitro as well as the survival of Escherichia coli cells exposed to antibiotics was carried out. Polyamines diminished the generation of superoxide in NADH/phenazine methosulfate reaction and neutralized H₂O₂ at least 20-fold less effectively than the antioxidants. Polyamines and antioxidants had no discernible effect on the antibacterial activity of β-lactam cefotaxime. Cadaverine reduced the decline in the CFU number caused by aminoglycoside amikacin by 2.7 orders of magnitude, while the other polyamines did by approximately 2 orders. The latter was 2 and 10 times higher than the effects of Trolox and thiourea, respectively. Under fluoroquinolone levofloxacin treatment, the protective effect of polyamines increased with an increase in their ability to bind DNA (cadaverine＜putrescine＜diaminopropane＜spermidine). Addition of spermidine resulted in an increase in the CFU number by 2 orders, which was 10 times lower than the effect of Trolox and 10 times higher than that of thiourea. The protective effect of polyamines on bacteria exposed to antibiotics is rather related to their ability to bind macromolecules including those targeted by antibiotics than to their properties to scavenge reactive oxygen species.
목차
Materials and Methods
Results and Discussion
References
참고문헌 (31)
참고문헌 신청
Das K and Misra H. 2004. Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines. Mol. Cell. Biochem. 262, 127?133.
Dwyer D, Belenky P, Yang J, MacDonald I, Martell J, Takahashi N, Chan C, Lobritz M, Braff D, Schwarz E, et al. 2014. Antibiotics induce redox-related physiological alterations as part of their lethality. Proc. Natl. Acad. Sci. USA 111, E2100?2109.
Flink I and Pettijohn D. 1975. Polyamines stabilise DNA folds. Nature 253, 62?63.
Gaboriau F, Vaultier M, Moulinoux J, and Delcros J. 2005. Antioxidative properties of natural polyamines and dimethylsilane analogues. Redox Rep. 10, 9?18.
Ha H, Sirisoma N, Kuppusamy P, Zweier J, Woster P, and Casero R. 1998. The natural polyamine spermine function directly as a free radical scavenger. Proc. Natl. Acad. Sci. USA 95, 11140?11145.
Dwyer D, Belenky P, Yang J, MacDonald I, Martell J, Takahashi N, Chan C, Lobritz M, Braff D, Schwarz E, et al. 2014. Antibiotics induce redox-related physiological alterations as part of their lethality. Proc. Natl. Acad. Sci. USA 111, E2100?2109.
Flink I and Pettijohn D. 1975. Polyamines stabilise DNA folds. Nature 253, 62?63.
Gaboriau F, Vaultier M, Moulinoux J, and Delcros J. 2005. Antioxidative properties of natural polyamines and dimethylsilane analogues. Redox Rep. 10, 9?18.
Ha H, Sirisoma N, Kuppusamy P, Zweier J, Woster P, and Casero R. 1998. The natural polyamine spermine function directly as a free radical scavenger. Proc. Natl. Acad. Sci. USA 95, 11140?11145.
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