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Purpose: The purpose of this study was to investigate the impact of sexual knowledge and attitudes on HPV vaccination intention among university students in D city. Methods: 213 of four-grade students were recruited from 2 universities in D city using convenience sampling. Data were collected through a self-administered questionnaire on sexual knowledge, sexual attitude, and HPV vaccination intention. IBM SPSS/WIN 21.0 program was used to analyze descriptive statistics, independent t-test, one-way ANOVA, Pearson correlation and step-wise regression. Results: Analysis showed that HPV vaccination intention was low and positively correlated with sexual knowledge(r=.29, p<.001) and sexual attitude(r=.27, p<.001). There was no correlation between sexual knowledge and sexual attitude(r=.09, p=.206). Factors affecting HPV vaccination intent were cervical cancer regular screening status(β=.33. p<.001), gender(β=-.22. p=.003) and sexual knowledge(β=-.16. p=.012). Cervical cancer regular screening status had the greatest influence on vaccination intent. These factors explained HPV vaccination intention(Adj. R2=.28). Conclusion: The finding of this study indicate that it is necessary to address sexual knowledge to increase HPV vaccination intention in university students. Further studies are required to investigate these variables with a larger size

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