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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Adipocytes are the main constituent of adipose tissue. Understanding the molecular basis of adipogenesis is pivotal to finding the therapeutic targets for treatment of obesity. Melatonin is associated with obesity and its mechanism is currently under intensive investigation. The objective of this study was to investigate the effect of melatonin on adipogenesis in differentiating preadipocytes. 3T3-L1 preadipocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 5% calf serum at 37 oC with 5% CO2 in a humidified incubator. Differentiation was induced using DMEM with 10% fetal bovine serum supplemented with MDI two days after cell confluence (day 0). Cells were treated with 0, 10 and 100 μM melatonin on either day 0 or day 5. 72 hours after each treatment, lipid accumulation was measured by oil red O staining. Proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to membranes. As a result, lipid accumulation decreased with melatonin treatment. ERK pathway, activated when differentiation is induced, also decreased with an increase in melatonin concentration. Furthermore, the expression of key adipogenic factors, C/EBPα, C/EBPβ, and PPARγ, were reduced by melatonin treatment. These results imply that melatonin may inhibit the process of adipogenesis and may have a role as a new anti-obesity agent.
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참고문헌 (31)
참고문헌 신청
Formiguera X / 2004 / Obesity : epidemiology and clinical aspects / Best Pract Res Clin Gastroenterol 18(6):1125~1146
Kopelman PG / 2000 / Obesity as a medical problem / Nature 404(6778):635
Flegal KM / 2012 / Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999-2010 / JAMA 307(5):491~497
Wellman NS / 2002 / Causes and consequences of adult obesity : health, social and economic impacts in the United States / Asia Pac J Clin Nutr 11:S705~S709
Itani O / 2017 / Short sleep duration and health outcomes : a systematic review, meta-analysis, and meta-regression / Sleep Med 32:246~256
Kopelman PG / 2000 / Obesity as a medical problem / Nature 404(6778):635
Flegal KM / 2012 / Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999-2010 / JAMA 307(5):491~497
Wellman NS / 2002 / Causes and consequences of adult obesity : health, social and economic impacts in the United States / Asia Pac J Clin Nutr 11:S705~S709
Itani O / 2017 / Short sleep duration and health outcomes : a systematic review, meta-analysis, and meta-regression / Sleep Med 32:246~256
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