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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 770 - 776 (7page)
이용수
초록· 키워드
오류제보하기
Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.
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참고문헌 (30)
참고문헌 신청
Kaufman PB / 1997 / A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein : implications for human nutrition and health / J. Altern. Complement Med 3:7~12
Morris PF / 1992 / Chemoattraction of zoospores of the soybean pathogen, Phytophthora sojae, by isoflavones / Physiol. Mol. Plant Pathol 40:17~22
Swinny EE / 2005 / Red clover Trifolium pratense L. phytoestrogens: UV-B radiation increases isoflavone yield, and postharvest drying methods change the glucoside conjugate profiles / J. Agric. Food Chem. 53:8273~8278
Rivera-Vargas LI / 1993 / Soybean flavonoid effects on and metabolism by Phytophthora sojae / Phytochemistry 32:851~857
Fang K / 2016 / Soy isoflavones and glucose metabolism in menopausal women : a systematic review and meta-analysis of randomized controlled trials / Mol. Nutr. Food Res 60:1602~1614
Morris PF / 1992 / Chemoattraction of zoospores of the soybean pathogen, Phytophthora sojae, by isoflavones / Physiol. Mol. Plant Pathol 40:17~22
Swinny EE / 2005 / Red clover Trifolium pratense L. phytoestrogens: UV-B radiation increases isoflavone yield, and postharvest drying methods change the glucoside conjugate profiles / J. Agric. Food Chem. 53:8273~8278
Rivera-Vargas LI / 1993 / Soybean flavonoid effects on and metabolism by Phytophthora sojae / Phytochemistry 32:851~857
Fang K / 2016 / Soy isoflavones and glucose metabolism in menopausal women : a systematic review and meta-analysis of randomized controlled trials / Mol. Nutr. Food Res 60:1602~1614
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