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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제4호
- 발행연도
- 2020.1
- 수록면
- 571 - 582 (12page)
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초록· 키워드
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Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal (Pestalotiopsis sydowiana PPR) extract was found to possess effective antipathogenic characteristics. The minimum inhibitory concentration (MIC) of the fungal extract against test pathogen Pseudomonas aeruginosa PAO1 was 1,000 μg/ml. Sub-MIC concentrations (250 and 500 μg/ml) of fungal extract reduced QSregulated virulence phenotypes such as the production of pyocyanin, chitinase, protease, elastase, and staphylolytic activity in P. aeruginosa PAO1 by 84.15%, 73.15%, 67.37%, 62.37%, and 33.65%, respectively. Moreover, it also reduced the production of exopolysaccharides (74.99%), rhamnolipids (68.01%), and alginate (54.98%), and inhibited the biofilm formation of the bacteria by 90.54%. In silico analysis revealed that the metabolite of P. sydowiana PPR binds to the bacterial QS receptor proteins (LasR and RhlR) similar to their respective natural signaling molecules. Cyclo(- Leu-Pro) (CLP) and 4-Hydroxyphenylacetamide (4-HPA) were identified as potent bioactive compounds among the metabolites of P. sydowiana PPR using in silico approaches. The MIC values of CLP and 4-HPA against P. aeruginosa PAO1 were determined as 250 and 125 μg/ml, respectively. All the antivirulence assays were conducted at sub-MIC concentrations of CLP (125 μg/ml) and 4-HPA (62.5 μg/ml), which resulted in marked reduction in all the investigated virulence factors. This was further supported by gene expression studies. The findings suggest that the metabolites of P. sydowiana PPR can be employed as promising QS inhibitors that target pathogenic bacteria.
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참고문헌 (56)
참고문헌 신청
Kalia VC / 2013 / Quorum sensing inhibitors : An overview / Biotechnol. Adv 31:224~245
Kalia VC / 2019 / Quorum sensing inhibitors as antipathogens : biotechnological applications / Biotechnol. Adv 37:68~90
Kalia VC / 2014 / In search of versatile organisms for quorum-sensing inhibitors : acyl homoserine lactones(AHL)-acylase and AHLlactonase / FEMS Microbiol. Lett 359:143~143
Kalia VC / 2014 / Microbes, antimicrobials and resistance : The battle goes on / Indian J. Microbiol 54:1~2
Kalia VC / 2011 / Quenching the quorum sensing system : potential antibacterial drug targets / Crit. Rev. Microbiol 37:121~140
Kalia VC / 2019 / Quorum sensing inhibitors as antipathogens : biotechnological applications / Biotechnol. Adv 37:68~90
Kalia VC / 2014 / In search of versatile organisms for quorum-sensing inhibitors : acyl homoserine lactones(AHL)-acylase and AHLlactonase / FEMS Microbiol. Lett 359:143~143
Kalia VC / 2014 / Microbes, antimicrobials and resistance : The battle goes on / Indian J. Microbiol 54:1~2
Kalia VC / 2011 / Quenching the quorum sensing system : potential antibacterial drug targets / Crit. Rev. Microbiol 37:121~140
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