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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제12호
- 발행연도
- 2019.1
- 수록면
- 2,006 - 2,013 (8page)
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The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
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참고문헌 (33)
참고문헌 신청
Morens DM / 2013 / Emerging infectious diseases : threats to human health and global stability / PLoS Pathog 9:e1003467
Morse SS / 1995 / Factors in the emergence of infectious diseases / Emerg. Infect. Dis 1:7~15
Smith KF / 2014 / Global rise in human infectious disease outbreaks / J. R. Soc. Interface 11:20140950
Fonkwo PN / 2008 / Pricing infectious disease, The economic and health implications of infectious diseases / EMBO Rep 9(Suppl 1):S13~S17
Christaki E / 2015 / New technologies in predicting, preventing and controlling emerging infectious diseases / Virulence 6:558~565
Morse SS / 1995 / Factors in the emergence of infectious diseases / Emerg. Infect. Dis 1:7~15
Smith KF / 2014 / Global rise in human infectious disease outbreaks / J. R. Soc. Interface 11:20140950
Fonkwo PN / 2008 / Pricing infectious disease, The economic and health implications of infectious diseases / EMBO Rep 9(Suppl 1):S13~S17
Christaki E / 2015 / New technologies in predicting, preventing and controlling emerging infectious diseases / Virulence 6:558~565
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