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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제10호
- 발행연도
- 2019.1
- 수록면
- 1,543 - 1,552 (10page)
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초록· 키워드
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Salmonella is a common zoonotic and foodborne pathogen that causes high morbidity and mortality in developing countries. In this study, we established and validated a polymerase spiral reaction (PSR) assay which targeted the conserved invasion gene (invA) of Salmonella by SYBR Green I indicator methods. Subsequently, assays for determination of the optimal conditions for optimal specificity and sensitivity of PSR were performed. We performed comprehensive evaluations using loop-mediated isothermal amplification (LAMP) and realtime PCR. A total number of 532 samples of daily food were analyzed by PSR. Twenty-seven bacterial strains were tested in the specificity assay, from which positive results were obtained only for 14-Salmonella strains. However, none of the 13 non-Salmonella strains was amplified. Similarly with LAMP and real-time PCR, the detection limit of the PSR assay was 50 CFU/ml. The PSR method was also successfully applied to evaluate the contamination with Salmonella in 532 samples of daily food, corroborating traditional culture method data. The novel PSR method is simple, sensitive, and rapid and provides new insights into the prevention and detection of foodborne diseases.
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참고문헌 (31)
참고문헌 신청
McClelland M / 2001 / Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 / Nature 413:852~856
Carvajal-Restrepo H / 2017 / Detection of Salmonella human carriers in Colombian outbreak areas / J. Infect. Dev. Ctries 11:228~233
Lopes ATS / 2018 / Multiplex real-time polymerase chain reaction for simultaneous quantification of Salmonella spp,, Escherichia coli, and Staphylococcus aureus in different food matrices: advantages and disadvantages / Biomed. Res. Int 2018:6104015
Vinayaka AC / 2019 / Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR / Biosens. Bioelectron 129:224~230
Hendriksen SW / 2004 / Animal-to-human transmission of Salmonella Typhimurium DT104A variant / Emerg. Infect. Dis 10:2225~2227
Carvajal-Restrepo H / 2017 / Detection of Salmonella human carriers in Colombian outbreak areas / J. Infect. Dev. Ctries 11:228~233
Lopes ATS / 2018 / Multiplex real-time polymerase chain reaction for simultaneous quantification of Salmonella spp,, Escherichia coli, and Staphylococcus aureus in different food matrices: advantages and disadvantages / Biomed. Res. Int 2018:6104015
Vinayaka AC / 2019 / Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR / Biosens. Bioelectron 129:224~230
Hendriksen SW / 2004 / Animal-to-human transmission of Salmonella Typhimurium DT104A variant / Emerg. Infect. Dis 10:2225~2227
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