지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 229 - 233 (5page)
이용수
초록· 키워드
오류제보하기
The anti-colorectal cancer monoclonal antibody CO17-1A (mAb CO), which recognizes the tumor-associated antigen EpCAM, was expressed in transgenic Arabidopsis plants. PCR and western blot analyses showed the insertion and expression of heavy chain (HC)/HC fused to the KDEL ER retention modif (HCK) and light chain (LC) of mAb CO and mAb CO with HCK (mAb COK) in Arabidopsis transformants. Both plantderived mAbP CO and mAbP COK were purified from a biomass of approximately 1,000 seedlings grown in a greenhouse. In sandwich ELISA, both mAbP CO showed a slightly higher binding affinity for the target, EpCAM, compared to mAbM CO. In cell ELISA, both mAbsP COs showed binding affinity to the human colorectal cancer cell line SW480. Furthermore, mAbM CO, mAbP CO, and mAbP COK exhibited dose and timedependent regression effects on SW480 cells in vitro. In summation, both mAbP CO and mAbP COK, expressed in Arabidopsis, recognized the target antigen EpCAM and showed anti-proliferative activity against human colorectal cancer cells.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Haggar FA / 2009 / Colorectal cancer epidemiology : incidence, mortality, survival, and risk factors / Clinics Colon Rect Surg 22:191
Moussavou G / 2015 / Production of monoclonal antibodies in plants for cancer immunotherapy / Bio Med Res Int 2015:306164
Ghislain Moussavou / 2018 / Baculovirus titration method based on MOI values for optimizing recombinant protein expression of the anti‐cancer vaccine candidate GA733‐Fc using Sf9 insect cells / Entomological Research 48(1):73~79
Song I / 2018 / Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody(mAb)CO17-1A affects mAb expression and plant stress response / PLoS One 13:e0198978
신천하 / 2019 / Immune response of heterologous recombinant antigenic protein of viral hemorrhagic septicemia virus (VHSV) in mice / Animal Cells and Systems 23(2):97~105
Moussavou G / 2015 / Production of monoclonal antibodies in plants for cancer immunotherapy / Bio Med Res Int 2015:306164
Ghislain Moussavou / 2018 / Baculovirus titration method based on MOI values for optimizing recombinant protein expression of the anti‐cancer vaccine candidate GA733‐Fc using Sf9 insect cells / Entomological Research 48(1):73~79
Song I / 2018 / Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody(mAb)CO17-1A affects mAb expression and plant stress response / PLoS One 13:e0198978
신천하 / 2019 / Immune response of heterologous recombinant antigenic protein of viral hemorrhagic septicemia virus (VHSV) in mice / Animal Cells and Systems 23(2):97~105
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
분자 비컨을 이용한 살아 있는 세포에서 단일클론항체 경쇄와 중쇄 mRNA 검출에 의한 세포주 선별방법
KSBB Journal
2016 .03
-
The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures
한국생물공학회 학술대회
2016 .04
-
Process intensification for Mab capture : Broth pretreatment and use of improved Protein A resin
한국생물공학회 학술대회
2019 .10
-
Mycobacterium abscessus D-alanyl-D-alanine dipeptidase induces the maturation of dendritic cells and promotes Th1-biased immunity
BMB Reports
2016 .01
-
Selective Capture EpCAM-Positive Cells with Antibody-conjugated Electrospun Polymer Nanofibers
한국생물공학회 학술대회
2019 .04
-
Cell-Free Expression of Snc1, An Arabidopsis Resistance Protein Snc1
한국생물공학회 학술대회
2017 .04
-
Identification and Evaluation of EpCAM Binding Endogenous Peptide of Panax Ginseng as Anticancerous Agent
한국생물공학회 학술대회
2023 .10
-
복어 균질액의 Caco-2 인간 결장직장암세포 성장 억제 효과에 대한 연구
KSBB Journal
2017 .09
-
Selective Capture and Culture of EpCAM-Positive Cells with Antibody-Conjugated Nanofibers
한국생물공학회 학술대회
2017 .10
-
Identification of m6A modification sites in Arabidopsis thaliana by using eXtreme Gradient Boosting
대한전자공학회 학술대회
2020 .08
-
계면활성제 EPA-073 및 전해질 NaOH를 첨가한 HCK슬러리와 HCK 원탄 슬러리의 점도 비교
한국에너지기후변화학회 학술대회
2015 .06
-
쿠웨이트 KNPC CFP MAB1 Project 소개
건설관리 : 한국건설관리학회 학회지
2016 .01
-
A New Family of Plant Antifungal Protein from Arabidopsis thaliana: Generation of Reactive Oxygen Species
한국생물공학회 학술대회
2017 .10
-
A human antibody against human endothelin receptor type A that exhibits antitumor potency
Experimental and Molecular Medicine
2021 .09
-
Cell Targeting Efficiency Comparison of Tumor Cell Receptor Binding and Cell Penetrating Peptide on the surface of human ferritin heavy chain
한국생물공학회 학술대회
2019 .04
-
Continuous Bioprocessing for MAb Manufacturing: A Myth Come Reality?
한국생물공학회 학술대회
2017 .04
-
Mab Manufacturing Bioprocessing: Current Landscape and Future Innovations
한국생물공학회 학술대회
2019 .04
-
밀리미터파 대역 5G 차량통신 네트워크에서 Multi-Armed Bandit 기반 빔 선택 기법
한국통신학회 학술대회논문집
2021 .02
-
Affinity Maturation of Anti-CD3 and Anti-EpCAM Antibody Fragments with Yeast Surface Display to Develop a Bispecific T-Cell Engager That Targets Brain Tumors
한국생물공학회 학술대회
2024 .09
-
2pACA and NAD⁺ Enhance Cell Growth and Productivity of Antibody-producing CHO Cells
한국생물공학회 학술대회
2021 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0