지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 366 - 376 (11page)
- DOI
- 10.7841/ksbbj.2019.34.4.366
이용수
초록· 키워드
오류제보하기
The discovery of the principle molecules involved in biosilicification both in diatoms and sponges brings out a new paradigm for silica synthesis at environmentally benign conditions. Here we introduce the biosynthetic method of silica using organic molecules for the undergraduate students. First, the biosilica synthesized was assessed through both a colorimetric method for quantification of silica and structural analysis by SEM image observation. Next, facile immobilization of an enzyme was demonstrated. Specifically, bovine carbonic anhydrase II (bCAII) was efficiently immobilized within silica matrix by simply adding itself to the reaction mixture containing polyamines and tetramethyl orthosilicate in phosphate buffer, obtaining 97% immobilization to initial amount of bCAII. The immobilized bCAII retained 40% of initial activity at 90℃ whereas free form did less than 10% of initial activity at 70℃ and the total yield from the 5 times reuses of immobilized bCAII was twice that of free bCAII. This experimental content that is hard to find in general biology or biochemistry lab could contribute to students’ interest in knowledge expansion and application of new materials.
목차
Abstract
1. INTRODUCTION
2. THEORETICAL BACKGROUND
3. RESEARCH METHODS
4. RESULTS AND DISCUSSION
5. CONCLUSION
REFERENCES
참고문헌 (39)
참고문헌 신청
Morello, J. A. / 2002 / Laboratory manual and workbook in microbiology / The McGraw-Hill
Boyer, R. F. / 2006 / Biochemistry laboratory: modern theories and techniques / Benjamin Cummings
Kroeger, N. / 1999 / Polycationic peptides from diatom biosilica that direct silica nanosphere formation / Science 286:1129~1132
Shimizu K. / 1998 / Silicatein alpha: cathepsin L-like protein in sponge biosilica / Proc. Natl. Acad. Sci. U. S. A 95:6234~6238
Tommasi, E. D. / 2018 / UV-shielding and wavelength conversion by centric diatom nanopatterned frustules / Sci. Rep 8:16285
Boyer, R. F. / 2006 / Biochemistry laboratory: modern theories and techniques / Benjamin Cummings
Kroeger, N. / 1999 / Polycationic peptides from diatom biosilica that direct silica nanosphere formation / Science 286:1129~1132
Shimizu K. / 1998 / Silicatein alpha: cathepsin L-like protein in sponge biosilica / Proc. Natl. Acad. Sci. U. S. A 95:6234~6238
Tommasi, E. D. / 2018 / UV-shielding and wavelength conversion by centric diatom nanopatterned frustules / Sci. Rep 8:16285
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UCI(KEPA) : I410-ECN-0101-2020-470-000264640