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논문 기본 정보

자료유형
학술저널
저자정보
Ahmad Ghorbani (Mashhad University of Medical Sciences) Hamid Reza Sadeghnia (Mashhad University of Medical Sciences) Amir Reza Afshari (North Khorasan University of Medical Sciences) Azar Hosseini (Mashhad University of Medical Sciences)
저널정보
한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.24 No.4
발행연도
2019.12
수록면
449 - 455 (7page)
DOI
10.3746/pnf.2019.24.4.449

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One of the major challenges for stem cell therapy of ischemic organs is that the transplanted cells are confronted with nutrient deficiency and oxidative stress. Previous studies have indicated that pretreatment of stem cells with cytoprotective phytochemicals improves their therapeutic potential. This study was aimed to investigate whether rosmarinic acid can enhance survival of adipose tissue-derived stem cells (ASCs) in nutrient-deficient culture as an in vitro model of ischemia. The ASCs were isolated from subcutaneous adipose tissue of male adult Wistar rats and incubated for 24 h with rosmarinic acid in nutrient-deficient (glucose- and serum-deprived, GSD) culture medium. In a separate experiment, ASCs were pre-incubated for 4 h with rosmarinic acid and then exposed to GSD conditions for 24 h. The viability of ASCs was determined using thiazolyl blue tetrazolium bromide assays. The effect of rosmarinic acid on the cell cycle was evaluated using propidium iodide staining. GSD conditions significantly decreased the viability of ASCs and enhanced the generation of reactive oxygen species (ROS), lipid peroxidation, sub-G1 cell populations, and necrosis. Both pre-incubation and incubation of ASCs with 0.75∼6 M rosmarinic acid significantly increased cell viability in GSD conditions. Rosmarinic acid further decreased the level of ROS, lipid peroxidation, the percent of cells in sub-G1 stage, and necrosis in GSD conditions. These findings suggest that rosmarinic acid enhances survival of ASCs cultured in nutrient-deficient conditions through promoting antioxidant effects. Therefore, rosmarinic acid may help preserve ASCs survival after they are transplanted into ischemic organs.
#necrosis #oxidative stress #rosmarinic acid #stem cells

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

참고문헌 (38)

참고문헌 신청
Amiri F / 2015 / In vitro augmentation of mesenchymal stem cells viability in stressful microenvironments : in vitro augmentation of mesenchymal stem cells viability / Cell Stress Chaperones 20:237~251 google schola Asadpour E / 2014 / Water-soluble compounds of lettuce inhibit DNA damage and lipid peroxidation induced by glucose/serum deprivation in N2a cells / Acta Pol Pharm 71:409~413 google schola Chamberlain G / 2007 / Concise review : mesenchymal stem cells : their phenotype, differentiation capacity, immunological features, and potential for homing / Stem Cells 25:2739~2749 google schola Dadashpour M / 2017 / Emerging importance of phytochemicals in regulation of stem cells fate via signaling pathways / Phytother Res 31:1651~1668 google schola Fallarini S / 2009 / Clovamide and rosmarinic acid induce neuroprotective effects in in vitro models of neuronal death / Br J Pharmacol 157:1072~1084 google schola

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