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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 132 - 138 (7page)
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초록· 키워드
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Among several marine-derived microorganisms isolated from the coast of Jeju Island that had antimicrobial activity against fish disease pathogens, Bacillus subtilis MD-02 was tested for its dietary effect on the innate immune response and disease resistance of olive flounder. Strain MD-02 was fed to the olive flounder at a concentration of 1.2 × 104, 1.2 × 106, or 1.2 × 108 CFU/100 g, respectively. Consequently, the hematocrit was higher in these three groups than that in the control group at 4 weeks, and the aspartate aminotransferase and alanine aminotransferase levels were decreased in the 1.2 × 108 and 1.2 × 104 CFU/100 groups compared with the control group levels. The amylase activity and total protein were significantly increased in the 1.2 × 104 CFU/100 g group at 3 weeks. The innate immune response, determined from the lysozyme and macrophage activities, was higher in the 1.2 × 108 CFU/100 g group than in the control group. In addition, treatment of the olive flounders with Streptococcus parauberis at 1.2 × 106 CFU/ml confirmed the mortality rate, which was 100% in the control group and 40−60% in the groups fed B. subtilis MD-02, indicating that the fish had resistance to fish disease pathogens. Therefore, it was confirmed that when fed MD-02, olive flounder builds an innate immune response and acquires resistance to fish disease pathogens, indicating that B. subtilis MD-02 can be developed as a beneficial feed additive.
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참고문헌 (41)
참고문헌 신청
Korean statistical information service / 2016 / Survey on the status of Fish Culture :38~39
이창훈 / 2007 / 제주지역 양식 넙치 (Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적 특성 / 한국어병학회지 20(1):33~40
Nguyen HT / 1999 / Selective agars for the isolation of Streptococcus iniae from Japanese flounder, Paralichthys olivaceus, and its cultural environment / J. Appl. Microbiol 86:769~776
Lee DC / 2001 / The study on the causal agent of streptoccosis (Lactococcus garvieae), isolated from cultured marine fishes / J. Fish Pathol 14:71~80
정용욱 / 2004 / 16S-23S rRNA Intergenic Spacer Region을 이용한 어류 병원성 Streptococcus iniae의 분자생물학적 동정 / 한국어병학회지 17(2):91~3
이창훈 / 2007 / 제주지역 양식 넙치 (Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적 특성 / 한국어병학회지 20(1):33~40
Nguyen HT / 1999 / Selective agars for the isolation of Streptococcus iniae from Japanese flounder, Paralichthys olivaceus, and its cultural environment / J. Appl. Microbiol 86:769~776
Lee DC / 2001 / The study on the causal agent of streptoccosis (Lactococcus garvieae), isolated from cultured marine fishes / J. Fish Pathol 14:71~80
정용욱 / 2004 / 16S-23S rRNA Intergenic Spacer Region을 이용한 어류 병원성 Streptococcus iniae의 분자생물학적 동정 / 한국어병학회지 17(2):91~3
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