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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제3호
- 발행연도
- 2019.1
- 수록면
- 482 - 488 (7page)
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초록· 키워드
오류제보하기
Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication. Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity. Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two recombinant proteins were tested in mice. The titers of antibodies were determined with a PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay. The IFN-γ response of immunized mice was measured by ELISA. The mice immunized with the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than mice injected with the Bac-Rep-Cap. IFN-γ in the Bac-Rep-Cap group was increased compared to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.
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참고문헌 (37)
참고문헌 신청
Allan GM / 2000 / Porcine circoviruses : a review / J. Vet. Diagn. Invest 12:3~14
An DJ / 2008 / Systemic cytokine profiles of mice vaccinated with naked DNAs encoding six open reading frame antigens of porcine circovirus type 2(PCV2) / Res. Vet. Sci 85:503~509
Blanchard P / 2003 / Protection of swine against postweaning multisystemic wasting syndrome(PMWS)by porcine circovirus type 2(PCV2)proteins / Vaccine 21:4565~4575
Cheung AK / 2003 / The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2 / Virology 313:452~459
Cheung AK / 2003 / Transcriptional analysis of porcine circovirus type 2 / Virology 305:168~180
An DJ / 2008 / Systemic cytokine profiles of mice vaccinated with naked DNAs encoding six open reading frame antigens of porcine circovirus type 2(PCV2) / Res. Vet. Sci 85:503~509
Blanchard P / 2003 / Protection of swine against postweaning multisystemic wasting syndrome(PMWS)by porcine circovirus type 2(PCV2)proteins / Vaccine 21:4565~4575
Cheung AK / 2003 / The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2 / Virology 313:452~459
Cheung AK / 2003 / Transcriptional analysis of porcine circovirus type 2 / Virology 305:168~180
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