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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한양대학교 세라믹공정연구센터 Journal of Ceramic Processing Research Journal of Ceramic Processing Research 제20권
- 발행연도
- 2019.1
- 수록면
- 63 - 69 (7page)
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초록· 키워드
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Quantification of viable spores is a time taking task due to the lack of rapid, efficient and accurate methods. This studypresented a simple spectrophotometric method for the detection of viable spores based on spore’s property of losing refractivityduring the germination process. By comparison of the results obtained by both spectrophotometric method and colonycounting method, a good linear correlation (R2 = 0.99) was achieved between viable spore concentration and OD loss underappropriate conditions. To avoid interference from ungerminable spores and vegetative cells, a turbidity complementationstrategy of keeping the initial concentration of spore suspensions at the same and relatively lower level was required. Thecalibration equation developed could be used to predict the viable spore yield produced in a series of fermentation experiments. The experimental results proved that this novel spectrophotometric method was sensitive, rapid, and easy to performcompared to conventional colony counting method.
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