지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 589 - 596 (8page)
이용수
초록· 키워드
오류제보하기
βPix is a guanine nucleotide exchange factor for the Rho family small GTPases, Rac1 and Cdc42. It is known to regulate focal adhesion dynamics and cell migration. However, the in vivo role of βPix is currently not well understood. Here, we report the production and characterization of βPix-KO mice. Loss of βPix results in embryonic lethality accompanied by abnormal developmental features, such as incomplete neural tube closure, impaired axial rotation, and failure of allantoischorion fusion. We also generated βPix-KO mouse embryonic fibroblasts (MEFs) to examine βPix function in mouse fibroblasts. βPix-KO MEFs exhibit decreased Rac1 activity, and defects in cell spreading and platelet-derived growth factor (PDGF)-induced ruffle formation and chemotaxis. The average size of focal adhesions is increased in βPix-KO MEFs. Interestingly, βPix-KO MEFs showed increased motility in random migration and rapid wound healing with elevated levels of MLC2 phosphorylation. Taken together, our data demonstrate that βPix plays essential roles in early embryonic development, cell spreading, and cell migration in fibroblasts.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Abercrombie, M. / 1970 / The locomotion of fibroblasts in culture / II. "RRuffling". Exp. Cell Res 60:437~444
Amano, M. / 1996 / Phosphorylation and activation of myosin by Rho-associated kinase(Rho-kinase) / J. Biol. Chem 271:20246~20249
Benard, V. / 2002 / Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods / Methods Enzymol 345:349~359
Bos, J. L. / 2007 / GEFs and GAPs : critical elements in the control of small G proteins / Cell 129:865~877
Campa, F. / 2006 / A new interaction between Abi-1 and betaPIX involved in PDGF-activated actin cytoskeleton reorganisation / Cell Res 16:759~770
Amano, M. / 1996 / Phosphorylation and activation of myosin by Rho-associated kinase(Rho-kinase) / J. Biol. Chem 271:20246~20249
Benard, V. / 2002 / Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods / Methods Enzymol 345:349~359
Bos, J. L. / 2007 / GEFs and GAPs : critical elements in the control of small G proteins / Cell 129:865~877
Campa, F. / 2006 / A new interaction between Abi-1 and betaPIX involved in PDGF-activated actin cytoskeleton reorganisation / Cell Res 16:759~770
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
AI-Based Dental Prostheses Fabrication Using Generative Adversarial Networks
Quantitative Bio-Science
2021 .05
-
Tropical cyclone surface wind retrieval using geostationary infrared satellite imagery and the pix2pix GAN algorithm
한국기상학회 학술대회 논문집
2024 .10
-
Endothelial Aquaporin-1 (AQP1) Expression Is Regulated by Transcription Factor Mef2c
Molecules and Cells
2016 .04
-
인공지능 기법을 활용한 태풍에 동반된 강수량의 보정
한국기상학회 학술대회 논문집
2021 .10
-
Conjugation element analysis and Brownian motion observation of magnetic nanoparticle structure conjugated with Corona-19 immune antibody
한국자기학회 학술연구발표회 논문개요집
2021 .11
-
Protective effects of P2X7 receptor deletion on aging-related memory alterations
Genes & Genomics
2016 .01
-
Phenotyping analysis of p53 knockout mice produced by gene editing and comparison with conventional p53 knockout mice
Genes & Genomics
2019 .01
-
Comparative N-Linked Glycan Analysis of Wild-Type and α1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches
Molecules and Cells
2015 .01
-
Supplement of High Protein-Enriched Diet Modulates the Diversity of Gut Microbiota in WT or PD-1H-Depleted Mice
Journal of Microbiology and Biotechnology
2021 .01
-
The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells
Molecules and Cells
2022 .11
-
Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells
Nutrition Research and Practice
2019 .04
-
Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells
Nutrition Research and Practice
2019 .04
-
Let-7c miRNA Inhibits the Proliferation and Migration of Heat-Denatured Dermal Fibroblasts Through Down-Regulating HSP70
Molecules and Cells
2016 .04
-
정상 및 atg5 유전자 제거 섬유아세포에서 자가포식체의 미세구조 및 이들의 정량적 분석
분석과학
2018 .10
-
The Probiotic Lactobacillus Prevents Citrobacter rodentium-Induced Murine Colitis in a TLR2-Dependent Manner
Journal of Microbiology and Biotechnology
2016 .01
-
해조류 추출물이 섬유아세포의 증식에 미치는 영향
한국해양바이오학회지
2015 .06
-
Vitamin C Promoted Liver Regeneration Following Partial Hepatectomy-induced Hepatic Injury in Senescence Marker Protein-30-deficient Mice
생명과학회지
2015 .03
-
Cooperative Interactions between Toll-Like Receptor 2 and Toll-Like Receptor 4 in Murine Klebsiella pneumoniae Infections
Journal of Microbiology and Biotechnology
2017 .01
-
Analytical method of agmatine and putrescine in human embryonic kidney cell by LC-ESI-MS/MS
한국분석과학회 학술대회
2019 .11
-
Dietary ellagic acid blocks inflammation-associated atherosclerotic plaque formation in cholesterol-fed apoE-deficient mice
Nutrition Research and Practice
2024 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0