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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제5호
- 발행연도
- 2019.1
- 수록면
- 696 - 703 (8page)
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초록· 키워드
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Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ΦCS01, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ΦCS01 has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ΦCS01 was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/ infected cell. Bacteriophage ΦCS01 was stable at 4–60°C for 1 h and lost infectivity after 1 h of heating at 70°C. Infectivity remained unaffected at pH 4–9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ΦCS01 DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ΦCS01 shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.
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참고문헌 (26)
참고문헌 신청
Yan QQ / 2012 / Cronobacter species(formerly known as Enterobacter sakazakii)in powdered infant formula : a review of our current understanding of the biology of this bacterium / J. Appl. Microbiol 113:1~15
Mofokeng L / 2011 / Characterization of Cronobacter species(Enterobacter Sakazakii)isolated from various South African food sources / J. Food Safety 31:98~107
Osaili TM / 2009 / Survival and growth of Cronobacter species(Enterobacter sakazakii)in wheat-based infant followon formulas / Lett. Appl. Microbiol 48:408~412
Greer GG / 2005 / Bacteriophage control of foodborne bacteria / J. Food Protect 68:1102~1111
Cai XQ / 2013 / Rapid detection and simultaneous genotyping of Cronobacter spp, (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis / PLos One 8:e67082
Mofokeng L / 2011 / Characterization of Cronobacter species(Enterobacter Sakazakii)isolated from various South African food sources / J. Food Safety 31:98~107
Osaili TM / 2009 / Survival and growth of Cronobacter species(Enterobacter sakazakii)in wheat-based infant followon formulas / Lett. Appl. Microbiol 48:408~412
Greer GG / 2005 / Bacteriophage control of foodborne bacteria / J. Food Protect 68:1102~1111
Cai XQ / 2013 / Rapid detection and simultaneous genotyping of Cronobacter spp, (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis / PLos One 8:e67082
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