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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 324 - 329 (6page)
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Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols.
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참고문헌 (23)
참고문헌 신청
Takahashi K / 2006 / Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors / Cell 126:663~676
Yamanaka S / 2007 / Strategies and new developments in the generation of patient-specific pluripotent stem cells / Cell Stem Cell 1:39~49
Okita K / 2007 / Generation of germline-competent induced pluripotent stem cells / Nature 448:313~317
Koren E / 2012 / Cell-penetrating peptides : breaking through to the other side / Trends Mol Med 18:385~393
El-Sayed A / 2009 / Delivery of macromolecules using arginine-rich cell-penetrating peptides : ways to overcome endosomal entrapment / AAPS J 11:13~22
Yamanaka S / 2007 / Strategies and new developments in the generation of patient-specific pluripotent stem cells / Cell Stem Cell 1:39~49
Okita K / 2007 / Generation of germline-competent induced pluripotent stem cells / Nature 448:313~317
Koren E / 2012 / Cell-penetrating peptides : breaking through to the other side / Trends Mol Med 18:385~393
El-Sayed A / 2009 / Delivery of macromolecules using arginine-rich cell-penetrating peptides : ways to overcome endosomal entrapment / AAPS J 11:13~22
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