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This study has performed to investigate the effects of CaCl₂ solution injection on artificial induction of antlerogenesis in female deer by breed. The rate of induction, yield of antler, length of antler, number of point, growth pattern of antler, and influence on the reproduction and regeneration of antler were investigated under treatments of 1ml, 1.5ml and 2ml of 30% CaCl₂ solution for six females each in sika deer, red deer and elk using injection onto the putative region of antlerogenesis. In sika deer, antlerogenesis was induced only in case of 1ml of CaCl₂ 30% solution, but was not induced in 1.5ml and 2ml. In red deer, antlerogenesis was induced in 2ml treatment, but was not induced in 1.5ml and 2ml. The rate of antlerogenesis was 8.3% in female sika deer and red deer altogether. In elk, antlerogenesis was induced in 1.5ml and 2ml but not in 1ml, and the rate of antlerogenesis was 67%. The antler which was induced in 1ml of 30 % CaCl₂ solution after 110 days was 21cm and 70g in sika deer, and 18.5cm� and 83g in red deer, respectively. The antler which was induced in 1.5ml of CaCl₂ 30% solution at March was average 334 ±3cm and 526~1008g, and the antler which was induced in 2ml of 30% CaCl₂ solution at July was average 32±1cm and 399~600g in elk. The average antler yields per female deer in 1.5ml and 2ml was 1,653±379g and 999g in spring and fall, respectively. The growth of antler by the CaCl₂ treatment in female elk was 0.5~1cm long after 20 days injection, 1~3cm after 40 days, and full-scale grew after 60 days. When the antler was cut after 120 days, the length was 28~44cm. The upper 70% of the antler was not ossificated. The shape of antler was determined not by the area of injection, but by method of injection. The induction of antlerogenesis did not influence on the reproduction at all, and regeneration of antlers in spring time was irregular, and the regeneration rate was 45% and the length and yield decreased as the cycle progressed. In conclusion, the optimum volume of 30% CaCl₂ solution for artificial induction of antlerogenesis in female elk was 1.5~2ml.

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