지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.
목차
등록된 정보가 없습니다.
참고문헌 (9)
참고문헌 신청
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Greenberg G L / 1997 / Convenient and controllable directview 3D imaging in conventional light microscopes: approaches via illumination and inspection / Proc. Roy. Microsc. Soc 32:87~100
Hama K / 1989 / Three-dimensional morphometrical study of dendritic spines of the granule cell in the rat dentate gyrus with HVEM stereo images / J. Electron. Microsc. Tech 12:80~87
Kiely C / 2011 / A new look at lunar soil collected from the sea of tranquility during the Apollo 11 mission / Microsc. Microanal 17:34~48
Greenberg G / 1993 / Novel method for stereo imaging in light microscopy at high magnifi cations / Neuroimage 1:121~128
Greenberg G L / 1997 / Convenient and controllable directview 3D imaging in conventional light microscopes: approaches via illumination and inspection / Proc. Roy. Microsc. Soc 32:87~100
Hama K / 1989 / Three-dimensional morphometrical study of dendritic spines of the granule cell in the rat dentate gyrus with HVEM stereo images / J. Electron. Microsc. Tech 12:80~87
Kiely C / 2011 / A new look at lunar soil collected from the sea of tranquility during the Apollo 11 mission / Microsc. Microanal 17:34~48
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