지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
High-voltage electron microscope (HVEM) has higher resolution and penetration power than conventional transmission electron microscope that could be load thick specimen.
Some researchers have taken this advantage of HVEM to explore 3-dimensional configuration of the biological structures including tissue and cells. Whole mount preparations has been employed to study some cell lines and primary culture cells. In this study, we would like to introduce useful whole mount preparation method for neuronal studies.
The plastic coverslips were punched, covered by formvar membrane and coated with carbon. The neurons obtained embryonic 18 rat hippocampus were seeded on the prepared cover slip. The coverslips were fixed, dried in freeze drier and kept in a descicator until HVEM observation.
We could observe detailed neuronal structures such as soma, dendrite and spine under HVEM without conventional thin section and heavy metal stain. The anaglyphic image based on stereo paired image (-8, +8) provides three dimensional perception of the neuronal dendrites and their spines.
This method could be applied to sophisticated analysis of dendritic spine under the various experimental conditions.
목차
등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Aizu S / 1981 / A simple method for whole-cell preparation in electron microscopy / J Microsc 124 124 (2) : 183 ~ 187
Goldstein S / 1984 / High-voltage electron microscopy of human diploid fibroblasts during ageing in vitro. Morphometric analysis of mitochondria / Exp Cell Res 154 (1) : 101
Hama K / 1994 / Three-dimensonal organizationof neuronal and glial processes: high voltage electron microscopy / Microsc Res Tech 29 (5) : 357 ~ 367
Kim IH / 2009 / Inositol 1,4,5-Trisphosphate 3-Kinase A Functions As a Scaffold for Synaptic Rac Signaling / JOURNAL OF NEUROSCIENCE 29 (44) : 14039 ~ 14049
Lee KJ / 2004 / Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy / NEUROSCI LETT 359 : 21-24 ~ 2
Goldstein S / 1984 / High-voltage electron microscopy of human diploid fibroblasts during ageing in vitro. Morphometric analysis of mitochondria / Exp Cell Res 154 (1) : 101
Hama K / 1994 / Three-dimensonal organizationof neuronal and glial processes: high voltage electron microscopy / Microsc Res Tech 29 (5) : 357 ~ 367
Kim IH / 2009 / Inositol 1,4,5-Trisphosphate 3-Kinase A Functions As a Scaffold for Synaptic Rac Signaling / JOURNAL OF NEUROSCIENCE 29 (44) : 14039 ~ 14049
Lee KJ / 2004 / Morphological analysis of spine shapes of Purkinje cell dendrites in the rat cerebellum using high-voltage electron microscopy / NEUROSCI LETT 359 : 21-24 ~ 2
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