개인구독
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples,cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, highpressure freezing allows for fixation of native non-pretreated samples up to 200 μm thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine,well preserved Arabidopsis stem’s inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.
목차
등록된 정보가 없습니다.
참고문헌 (6)
참고문헌 신청
Beckett ND / 1986 / Low-temperature scanning electron microscopy. Ultrastructural Techniques for Microorganisms / Plenum Publishing Corporation : 45 ~ 86
Boyde A / 1972 / Biological specimen preparation for the scanning electron microscope: an overview / Proceedings of the Fifth Annual Electron Microscopy Symposium
Echlin P / 1986 / The preparation and X-ray microanalysis of bulk frozen hydrated vacuolated plant tissue / J Microsc 141 : 329 ~ 348
Sargent JA / 1988 / Low temperature scanning electron microscopy: advantages and applications / Scanning Microsc 2 : 835 ~ 849
Shimoni E / 1998 / On optimizing high-pressure freezing: from heat transfer theory to a new microbiopsy device / J Microsc 192 : 236 ~ 247
Boyde A / 1972 / Biological specimen preparation for the scanning electron microscope: an overview / Proceedings of the Fifth Annual Electron Microscopy Symposium
Echlin P / 1986 / The preparation and X-ray microanalysis of bulk frozen hydrated vacuolated plant tissue / J Microsc 141 : 329 ~ 348
Sargent JA / 1988 / Low temperature scanning electron microscopy: advantages and applications / Scanning Microsc 2 : 835 ~ 849
Shimoni E / 1998 / On optimizing high-pressure freezing: from heat transfer theory to a new microbiopsy device / J Microsc 192 : 236 ~ 247
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