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This study aimed to develop analytical method for 8-isoprostanes as biomarkers for oxidative stress with LC/MS/MS technique and to apply the method for human urine samples. Analyzed compounds for urinary oxidative stress markers were 7 stereo-isomers of prostaglandins and the internal standard (iso-PGF2α-d4) was used to adjust the recovery rate.
The method for determining urinary iso-PGF2α consisted of solid phase extraction and LC/MS/MS detection. Separation of isomers of prostaglandins completed by porous graphitic carbon column and buffer solution. Detection limits for urinary markers of oxidative stress, iso-PGF2α with LC/MS/MS were 0.01 ng/ml by S/N ratio 3 and 0.028 ng/ml by calculated as to FDA method. The recovery (92.8~101.9%) and precision (8.8~20.7%) of analysis were feasible for detecting iso-PGF2α in real human urine samples. We detected 4 isomers of prostaglandins in human urine samples.
Mean (standard deviation) of urinary iso-PGF2α concentration were 0.231 (0.117) ng/mg creatinine for smoking group and 0.154 (0.082) ng/mg creatinine for non-smoking group.
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참고문헌 (23)
참고문헌 신청
Ahmadzadehfar, H / 2006 / Passive cigarette smoking increases isoprostane formation / Life Sciences 78 (8) : 894 ~ 897
Block, G / 2002 / Factors associated with oxidative stress in human populations / American Journal of Epidemiology 156 (3) : 274 ~ 285
Cavalca, V / 2010 / Simultaneous quantification of 8-iso-prostaglandin-F2α and 11-dehydro thromboxane B2 in human urine by liquid chromatography tandem mass spectrometry / A
Devaraj, S / 2001 / Divergence between LDL oxidative susceptibility and urinary F2-isoprostanes as measures of oxidative stress in type 2 diabet
Dimopoulos, N / 2008 / Increased plasma levels of 8-iso-PGF2α and IL-6 in an elderly population with depression / Psychiatry Research 161 : 59 ~ 66
Block, G / 2002 / Factors associated with oxidative stress in human populations / American Journal of Epidemiology 156 (3) : 274 ~ 285
Cavalca, V / 2010 / Simultaneous quantification of 8-iso-prostaglandin-F2α and 11-dehydro thromboxane B2 in human urine by liquid chromatography tandem mass spectrometry / A
Devaraj, S / 2001 / Divergence between LDL oxidative susceptibility and urinary F2-isoprostanes as measures of oxidative stress in type 2 diabet
Dimopoulos, N / 2008 / Increased plasma levels of 8-iso-PGF2α and IL-6 in an elderly population with depression / Psychiatry Research 161 : 59 ~ 66
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