지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 561 - 567 (7page)
이용수
초록· 키워드
오류제보하기
Objectives: Parabens are widely used as antimicrobial agents in pharmaceuticals and cosmetics as well as by the food industry. Parabens have been reported to show weak estrogenic activity and be related to health effects such as allergic reactions and skin and breast cancer. We evaluated an online solid phase extraction (SPE) method coupled with LC-MS/MS technique using free and conjugated parent parabens in human urine for assessing human exposure to parabens.
Methods: We employed LC/MS/MS through online solid phase extraction and column-switching techniques and analyzed free and conjugated parabens as biomarkers of human exposure. Four major parabens, methylparaben (MP), ethyl-paraben (EP), propyl-paraben (PP) and butyl-paraben (BP), were analyzed. Method validation was performed by sensitivity, accuracy, precision and comparison of the results of online SPE with offline SPE.
Results: The limits of detection (LOD) were in the range of 0.2-2 ng/mL, and actual limits of quantification (LOQ) were in the range of 0.7-6 ng/mL urine, depending upon the compound. Accuracy was in the range of 98.3-106.4%, and precision was in the range of 1.3-8.7% (CV) depending upon the compound. We found a good correlation between the results of analysis by online SPE method and that by off-line SPE method.
Conclusions: The online SPE method showed proper LOD and validated accuracy, precision and good correlation with the offline method for analyzing parabens in urine.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
/ 2012 / Danish center on endocrine disrupters, Evaluation of tebuconazole, triclosan, methylparaben and ethylparaben according to the Danish proposal for criteria for endocrine disrup
Boberg J / 2010 / Possible endocrine disrupting effects of parabens and their metabolites / Reproductive Toxicology 30 : 301 ~ 312
Casas L / 2011 / Urinary concentrations of phthalates and phenols in a population of Spanish, pregnant women and children / Environment International 37 : 858 ~ 866
Darbre PD / 2004 / Concentrations of Parabens in Human Breast Tumours / J. Appl. Toxicol 24 : 5 ~ 13
FDA / 1998 / Validation of analytical procedures: Methodology, recommended for implementation at step 7 of the VICH process on 22 October 1998 by the VICH steering committee(64. guida
Boberg J / 2010 / Possible endocrine disrupting effects of parabens and their metabolites / Reproductive Toxicology 30 : 301 ~ 312
Casas L / 2011 / Urinary concentrations of phthalates and phenols in a population of Spanish, pregnant women and children / Environment International 37 : 858 ~ 866
Darbre PD / 2004 / Concentrations of Parabens in Human Breast Tumours / J. Appl. Toxicol 24 : 5 ~ 13
FDA / 1998 / Validation of analytical procedures: Methodology, recommended for implementation at step 7 of the VICH process on 22 October 1998 by the VICH steering committee(64. guida
이 논문의 저자 정보
최근 본 자료
댓글(0)
0