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논문 기본 정보
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- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제17권 제2호
- 발행연도
- 2008.1
- 수록면
- 189 - 195 (7page)
이용수
초록· 키워드
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The sequence of hydroxypyruvate isomerase gene was
obtained in NCBI. In this study, the hydroxypyruvate
isomerase gene of Bombyx.mori was identified and
annotated with bioinformatics tools. The result was
confirmed by RT-PCR, prokaryotic expression, mass
spectrographic analysis and sub-cellular localization.
The hydroxypyruvate isomerase cDNA comtains a
783bp ORF, and has 4 exons. The deduced protein has
260 amino acid residues with the predicted molecular
weight of 29169.30 Da, isoelectric point of 6.10, and
contains conserved PRK09997 and Hfi domains. The
hydroxypyruvate isomerases of Nasonia vitripennis
and Bombyx mori have a high homology. Through RTPCR
analysis, we found that this transcript was
present in testis, ovary, blood-lymph, fat body, midgut,
silk gland and tuba Malpighii. This protein was
located in cytoplasm through immunohistochemistry.
We submitted the cloned gene under the accession
number EU344910. The enzyme has been classified
under accession number EC 5.3.1.22.
목차
등록된 정보가 없습니다.
참고문헌 (8)
참고문헌 신청
Ashiuchi, M. / 1999 / Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase / Biochim Biophys Acta 1435 : 153 ~ 159
Braun, W. / 1979 / Untersuchungen zum Glyoxylsaiurestoffwechsel von Bacillus fastidiosus Stamm 83* / Arch. Microbiol 121 : 129 ~ 134
Chen, H. Q. / 2007 / Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus / FEBS Lett 581 : 5836 ~ 5842
Kumar, S. / 2004 / MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment / Brief Bioinform 5 : 150 ~ 163
Lambert, C. / 2002 / ESyPred3D: Prediction of proteins 3D structures / Bioinformatics 18 : 1250 ~ 1256
Braun, W. / 1979 / Untersuchungen zum Glyoxylsaiurestoffwechsel von Bacillus fastidiosus Stamm 83* / Arch. Microbiol 121 : 129 ~ 134
Chen, H. Q. / 2007 / Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus / FEBS Lett 581 : 5836 ~ 5842
Kumar, S. / 2004 / MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment / Brief Bioinform 5 : 150 ~ 163
Lambert, C. / 2002 / ESyPred3D: Prediction of proteins 3D structures / Bioinformatics 18 : 1250 ~ 1256
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