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논문 기본 정보
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- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제25권 제1호
- 발행연도
- 2012.1
- 수록면
- 111 - 114 (4page)
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In previous studies we have shown that a sw17255gene was expressed in hemocyte-specific tissues of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae).
It was verified that the sw17255 core promoter region contains elements that regulate the expression of this gene in hemocyte tissue; the selected promoter region spans nucleotides -1 to -2,112 upstream of the start codon. Each of the luciferase reporter gene expression vectors under the control of 4 different kinds of promoter candidates, (-2,112/-1), (-1,640/-1), (-1,169/-1) and (-579/-1), and the control reporter plasmid DNA, were introduced into B. mori larval coelom by direct injection using a syringe. The promoter candidate [E] (-579/-1) showed more than 1.67 fold transcriptional activity compared to control promoter activity. Higher productivity of an expressed gene in the transgenic silkworm by this promoter combination could be achieved in the near future. The foreign recombinant protein could be easily harvested from the blood of the transgenic silkworm.
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참고문헌 (14)
참고문헌 신청
Abraham EG / 2005 / Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements / Insect Mol Biol 14 : 271
Chen XG / 2007 / The Anopheles gambiae vitellogenin gene (VGT2) promoter directs persistent accumulation of a reporter gene product in transgenic Anopheles stephensi followin
Cong L / 2011 / Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms / Biochem Bioph Res Co 410 : 721 ~ 725
Garcia AC / 2011 / Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila / BMC Mol Biol 12 : 32 ~
Juven-Gershon T / 2008 / The RNA polymerase II core promoter-the gateway to transcription / Current Opinion Cell Biology 20 : 253 ~ 259
Chen XG / 2007 / The Anopheles gambiae vitellogenin gene (VGT2) promoter directs persistent accumulation of a reporter gene product in transgenic Anopheles stephensi followin
Cong L / 2011 / Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms / Biochem Bioph Res Co 410 : 721 ~ 725
Garcia AC / 2011 / Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila / BMC Mol Biol 12 : 32 ~
Juven-Gershon T / 2008 / The RNA polymerase II core promoter-the gateway to transcription / Current Opinion Cell Biology 20 : 253 ~ 259
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