지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제23권 제2호
- 발행연도
- 2011.1
- 수록면
- 231 - 238 (8page)
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Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, Papilio xuthus. A full length cDNA of P. xuthus attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5’ RACE.
The complete P. xuthus attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence,a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of P. xuthus attacin showed 48-52% and 24-30%identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of P. xuthus attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature P. xuthus attacin into the expression vector, highly expressed in E. coli BL21 cells, and its antibacterial activity was analyzed. Recombinant P.
xuthus attacin evidenced considerably antibacterial activity against Gram-negative bacteria, E. coli ML 35and Klebsiella pneumonia.
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참고문헌 (20)
참고문헌 신청
Asling B / 1995 / Identification of early genes in the Drosophila immune response by PCRbased differential display: the attacin A gene and the evolution of attacin-like prote
Carlsson A / 1991 / Attacin, an antibacterial protein from Hyalophora cecropia, inhibits synthesis of outer membrane proteins in Escherichia coli by interfering with omp gene
Carlsson A / 1998 / Attacinan insect immune protein-binds LPS and triggers the specific inhibition of bacterial outer-membrane synthesis / Microbiol 144 : 2179 ~ 2188
Dushay MS / 2000 / Two attacin antibacterial genes of Drosophila melanogaster / Gene 246 : 49 ~ 57
Engstram P / 1984 / The antibacterial effect of attacins from the silk moth Hyalophora cecropia is directed against the outer membrane of Escherichia coli / EMBO J 3 : 3347 ~
Carlsson A / 1991 / Attacin, an antibacterial protein from Hyalophora cecropia, inhibits synthesis of outer membrane proteins in Escherichia coli by interfering with omp gene
Carlsson A / 1998 / Attacinan insect immune protein-binds LPS and triggers the specific inhibition of bacterial outer-membrane synthesis / Microbiol 144 : 2179 ~ 2188
Dushay MS / 2000 / Two attacin antibacterial genes of Drosophila melanogaster / Gene 246 : 49 ~ 57
Engstram P / 1984 / The antibacterial effect of attacins from the silk moth Hyalophora cecropia is directed against the outer membrane of Escherichia coli / EMBO J 3 : 3347 ~
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