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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제23권 제1호
- 발행연도
- 2011.1
- 수록면
- 147 - 153 (7page)
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The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins.
We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h,respectively, for the expression of both gC and gD in E.
coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.
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참고문헌 (22)
참고문헌 신청
Assadi-Porter FM / 2008 / Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli / Protein Expr Purif 58
Balasch M / 1998 / Aujeszky's disease (pseudorabies) virus detection in cerebrospinal fluid in experimentally infected pigs / Vet Microbiol 60 (4) : 99 ~ 106
Bane SE / 2007 / Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli / Protein Expr Purif 52 (2) : 348 ~ 355
Burgess-Brown NA / 2008 / Codon optimization can improve expression of human genes in Escherichia coli: A multigene study / Protein Expr Purif 59 (1) : 94 ~ 102
Grabowska AK / 2009 / New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection / Vaccine 27 (27) : 3584
Balasch M / 1998 / Aujeszky's disease (pseudorabies) virus detection in cerebrospinal fluid in experimentally infected pigs / Vet Microbiol 60 (4) : 99 ~ 106
Bane SE / 2007 / Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli / Protein Expr Purif 52 (2) : 348 ~ 355
Burgess-Brown NA / 2008 / Codon optimization can improve expression of human genes in Escherichia coli: A multigene study / Protein Expr Purif 59 (1) : 94 ~ 102
Grabowska AK / 2009 / New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection / Vaccine 27 (27) : 3584
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