지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제22권 제2호
- 발행연도
- 2011.1
- 수록면
- 83 - 93 (11page)
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Biochemical and enzymatic characterization for extracellular protease isolated from Cordyceps militaris cultivated on rice bran medium was investigated. C militaris produced proteolytic enzymes from 10 days after inoculation, maximum enzyme production was found at 25 days. The optimum temperature and pH of proteases production was at 25℃ and pH 7.0,respectively. The protease activity was observed in the four peaks (Pro-I, Pro-II, Pro-III, and Pro-IV) separated through Sephadex G-100 column chromatography.
The separated protease was optimally active at 25℃. Optimum pH of the protease was between 7 and 8. Enzyme was also stable over at 30-80℃. The enzyme was highly stable in a pH range of 4-9. Protease activity was found to be slightly decreased by the addition of Mg^(2+), Mn^(2+), Zn^(2+), Fe^(2+) and Cu^(2+), whereas inhibited by the addition of Ca^(2+) and Co^(2+) Protease activity was inhibited by protease inhibitor PMSF. On the other hand, the partially purified protease was investigated on proteolytic protease activity by zymogram gel electrophoresis using three substances (casein, gelatin and fibrin). Four active bands (F-I, FII,F-III, and F-IV) of fibrin degradation were revealed on fibrin zymogram gels. Both of F-II and FIII showed caseinolytic, fibrinolytic and gelatinolytic activities in three gels. Thermostability, pH stability,and pH-thermostability of the enzyme determined the residual fibrinolytic activity also displayed on fibrin zymogram gel. The only one enzyme (F-II) displayed over a broad range of temperature at 30-90℃. The FII displayed fibrinolytic activity in the pH range 3-5,but was inactivated in the range of pH 6-11. The F-I and F-III showed enzyme activity in the pH range of 6-11. In the pH-thermostability, the F-II only kept fibrinolytic activity after heating at 100℃ for 10, 20and 30 min at pH 3 and pH 7, respectively. On the other hand, the F-II was retained activity until heating for 10 min under pH 11 condition. By using fibrin zymogram gel electrophoresis, extracellular fibrinolytic enzyme F-II from C. militaris showed unusual thermostable under acid and neutral conditions.
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참고문헌 (24)
참고문헌 신청
Alicja S / 1998 / Towards an integrated management of Dendrolimus pini L. Proceedings; in Population dynamics, impacts, and integrated management of forest defoliation insects : 129 ~ 14
Anderson SO / 1985 / Sclerotization and tanning of the cuticle; in Comprehensive insect physiology, biochemistry and pharmarcology / Oxford : 59 ~ 74
Bidochka MJ / 1987 / Purification and properties of an extracellular protease produced by the entomopathegenic fungus Beauveria bassiana / Appl and Environ Microbiol 53 : 167
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Braun V / 1980 / Excretion of a protease by Serratia marcescens / Tech Microbiol 124 : 55 ~ 61
Anderson SO / 1985 / Sclerotization and tanning of the cuticle; in Comprehensive insect physiology, biochemistry and pharmarcology / Oxford : 59 ~ 74
Bidochka MJ / 1987 / Purification and properties of an extracellular protease produced by the entomopathegenic fungus Beauveria bassiana / Appl and Environ Microbiol 53 : 167
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Braun V / 1980 / Excretion of a protease by Serratia marcescens / Tech Microbiol 124 : 55 ~ 61
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