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The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT)to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the lowability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation tooviposition in artificial substrates. In the present study, fruit grapes were used as an alternativeegg collection medium to harvest eggs and young larvae from freshly colonized wild strainsoriginating from France, Italy, Spain and Croatia. The larvae were allowed to develop into thefruits until the second instar, before they were extracted out and further reared on a standardartificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles tooviposit. Finally, the performance of hybrid strains created from crosses between wild andlong colonised flies was assessed. The results showed that females of all 4 wild strainsreadily oviposited eggs in grapes and from the F2 generation onward, females from all strainswere adapted to laying eggs in wax bottles. No difference was observed in eggs and pupaeproduction among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

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