지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.
목차
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참고문헌 (17)
참고문헌 신청
Cardle L / 2000 / Computational and experimental characterization of physically clustered simple sequence repeats in plants / Genetics 156 : 847 ~ 854
Ferguson ME / 2004 / Microsatellite identification and characterization in peanut (A. hypogaea L.) / Theor Appl Genet 108 : 1064 ~ 1070
Hao Cui / 2010 / Genetic diversity and population structure of rice accessions from South Asia using SSR markers / 한국육종학회지 42 (1) : 11 ~ 22
He G / 1997 / Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.) / Euphytica 97 : 143 ~ 149
Jung Yoon Yi / 2010 / Phylogenetic analysis and association of markers and traits related to starch contents in Korean potato cultivars using SSRs / 한국육종학회지 42 (1) :
Ferguson ME / 2004 / Microsatellite identification and characterization in peanut (A. hypogaea L.) / Theor Appl Genet 108 : 1064 ~ 1070
Hao Cui / 2010 / Genetic diversity and population structure of rice accessions from South Asia using SSR markers / 한국육종학회지 42 (1) : 11 ~ 22
He G / 1997 / Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.) / Euphytica 97 : 143 ~ 149
Jung Yoon Yi / 2010 / Phylogenetic analysis and association of markers and traits related to starch contents in Korean potato cultivars using SSRs / 한국육종학회지 42 (1) :
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