지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
A resveratrol synthase (RS) gene was isolated from peanut (Arachis hypogaea, L. cv. Jinpoong) plants. This gene was
placed under the control of the cauliflower mosaic virus 35S promoter (CaMV35S) and introduced into two Korean varieties of
potato (Solanum tuberosum L. cvs. Jasim and Jowon) plants by Agrobacterium-mediated gene transfer. Putative transformants were
screened by PCR with primers designed from CaMV 35S promoter, NOS terminator and RS gene. Most of selected transgenic
potato plants showed the amplification of expected fragments by PCR of genomic DNA with gene-specific primers, while they
were absent in untransformed control plants. Expression of the resveratrol synthase gene was also examined by northern blot analysis.
The transformants showed a band which was lacking in the control plant, confirming that the introduced gene is transcribed
into mRNA in the transformants. The strength of the band, which reflected the level of mRNA expression, differed among the
individual transformants. Among the transformants obtained, the highest trans-resveratrol content in the transgenic young leaves
of purple-fleshed “Jashim” was 2.11 μgg-1 fresh weight and that in the microtubers in vitro of purple fleshed “Jashim” was 8.31 μgg-1 fresh weight. This amount of resveratrol may have a positive biological effect on human health.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
An G / 1987 / Binary Ti vectors for plant transformation and promoter analysis / Methods Enzymol. 153 : 292 ~ 305
An G / 1993 / Organization of the genes encoding chalcone synthase in Pisum sativum / Plant Mol. Biol. 21 : 789 ~ 803
Baek SH / 2006 / Development of transgenic rice producing resveratrol / Korean J of Crop Science 51 : 141 ~
Bergmann H / 1999 / Activation of stress resistance in plants and consequences for product quality / Journal of Applied Botany 73 : 153 ~ 161
Bilodeau P / 1999 / A prolonged cold treatment-induced cytochrome P450 gene from Arabidopsis thaliana / Plant Cell Environ 22 : 791 ~ 800
An G / 1993 / Organization of the genes encoding chalcone synthase in Pisum sativum / Plant Mol. Biol. 21 : 789 ~ 803
Baek SH / 2006 / Development of transgenic rice producing resveratrol / Korean J of Crop Science 51 : 141 ~
Bergmann H / 1999 / Activation of stress resistance in plants and consequences for product quality / Journal of Applied Botany 73 : 153 ~ 161
Bilodeau P / 1999 / A prolonged cold treatment-induced cytochrome P450 gene from Arabidopsis thaliana / Plant Cell Environ 22 : 791 ~ 800
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Transfer of Peanut Resveratrol Synthase Gene to Potato (Solanum tuberosum L.)
한국원예학회 학술발표요지
2005 .05
-
Resveratrol Analysis of Transgenic Lettuce (Lactuca sativa) Transformed with Stilbene Synthase Gene from Chinese Cabbage
한국원예학회 학술발표요지
2009 .10
-
Resveratrol의 항산화 및 산화촉진 활성이 세포독성에 미치는 영향
한국식품과학회지
2011 .02
-
Development of Efficient Transformation System and Transgenic Plants with Stilbene Synthase (Stsy) Gene in Oriental Melon
한국원예학회 기타 간행물
2006 .08
-
Molecular Cloning and Expression of Dihydroflavonol 4-reductase Gene in Tuber Organs of Purple-fleshed Potatoes
Applied Biological Chemistry
2006 .01
-
두 가지 제초제에 대하여 저항성을 가지는 항생제 마커-프리 형질전환 감자 육성
Journal of Plant Biotechnology
2007 .01
-
Resveratrol의 첨가가 육계의 생산성, 혈액 생화학 특성, 혈액 내 면역글로불린과 혈액 내 항산화 인자에 미치는 영향
한국가금학회지
2015 .06
-
Primary 인체 전립선 암세포에서 Resveratrol의 Apoptosis 유도 효과
한국식품영양과학회지
2010 .08
-
Agrobacterium-Mediated Transformation of Rape (Brassica napus) with Phytoene Synthase
HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY
2006 .06
-
Expression of Human Lactoferrin Gene in Transgenic Rice(Oryza sativa L.)
Journal of Plant Biotechnology
2007 .01
-
식이 폴리페놀 성분 resveratrol과 일반의약품의 복합처리에 의한 간 및 장관계 세포독성 평가
한국식품과학회지
2010 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0