지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Citrus is one of the major fruits produced in Korea. There are about 20 species mainly grown in Jeju Island, Korea.
Four representative species, which are quite different in the shape of leaf and the taste of fruit, were selected and were used to profile the transcriptomes. These species are ‘Miyagawa Wase’ (C. unshiu Marcov.) satsuma mandarin, ‘Kiyomi’ (C. unshiu Marcov. × C. sinensis) mandarin hybrid, ‘Dangyuja’ (C. grandis) and ‘Natsudaidai’ (C. natsudaidai). Classification of the up-regulated and down-regulated genes using the Cluster of Orthologous Groups of proteins (COG) database reveals that the number of genes included in each group differed significantly among the four species. Several genes that showed significant differences in expression on the microarray were selected and their expression patterns were examined by reverse transcriptionploymerase chain reaction. Metabolic genes such as tyrosine decarboxylase and β-glucosidase ligase were found to be highly expressed in Miyagawa Wase, relative to other species. On the other hand, the expression level of mannose phosphate isomerase was lower in Miyagawa Wase. An efflux pump gene was found to be up-regulated in Kiyomi, whereas cinnamyl-alcohol dehydrogenase was down-regulated. β-carotene 15,15’-dioxygenase, which is involved in the vitamin metabolism, was up-regulated in Natsudaidai. Interspecific differentiations of gene expression are analyzed in terms of the metabolic pathways and their possible roles in citrus species.
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참고문헌 (22)
참고문헌 신청
Agusti J / 2008 / Ethylene-induced differential gene expression during abscission of citrus leaves / J Exp Bot 59 : 2717 ~ 2733
Akakabe Y / 2010 / A comparison of volatile components of Setomi with its parent cultivars / Biosci. Biotechnol Biochem 74 : 659 ~ 662
Alos E / 2008 / An evaluation of the basis and consequences of a stay-green mutation in the navel orange citrus mutant using transcriptomic and proteomic profiling and metabo
Bartley GE / 2010 / PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus / New Biotechnol 27 : 308 ~ 316
Bernardi J / 2010 / Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening / Plant Physiol 167 : 301 ~ 310
Akakabe Y / 2010 / A comparison of volatile components of Setomi with its parent cultivars / Biosci. Biotechnol Biochem 74 : 659 ~ 662
Alos E / 2008 / An evaluation of the basis and consequences of a stay-green mutation in the navel orange citrus mutant using transcriptomic and proteomic profiling and metabo
Bartley GE / 2010 / PCR amplification and cloning of tyrosine decarboxylase involved in synephrine biosynthesis in Citrus / New Biotechnol 27 : 308 ~ 316
Bernardi J / 2010 / Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening / Plant Physiol 167 : 301 ~ 310
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