지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Soybean is one of the most widely planted crops supplying oil and edible bean. Soybean has a complicated genome due to at least two rounds of polyploidization. Although there are several types of molecular markers available for soybean genotyping, agarose gel-based molecular markers for this purpose are not sufficient. The objective of this study was to develop STS markers that can discriminate Korean soybean cultivars using agarose gels. We employed methylation filtering (MF)technique for constructing a genomic DNA library of “HwangKeum” and selected 1,440 colonies. Of the 1,440 colonies, 887colonies which had more than 700 bp of insert DNA were sequenced. A total of 143 STS primer sets were designed from sequencing data of selected colonies. One hundred one STS primer sets showed unique band in 14 Korean soybean cultivars and digested with 7 restriction enzymes including Hinf I. Among them, 18 STS primer sets showed polymorphic bands. We developed 51 marker combinations and selected 14 marker combinations, which were used to discriminate 110 Korean soybean cultivars.
As the results, 106 cultivars (96.4%) were discriminated by using agarose gel-based markers. Therefore, STS-CAPS markers could be useful to discriminate soybean cultivars and identify soybean genotypes.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bang KH / 2010 / Construction of genomic DNA library of Korean ginseng (panax ginseng C.A. Meyer) and development of sequence-tagged sites / Biol. Pharm. Bull 33 : 1579 ~ 158
Bennetzen JL / 1994 / Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA / Genome 37 : 565 ~ 576
Gruenbaum Y / 1981 / Sequence specificity of methylation in higher plant DNA / Nature 292 : 860 ~ 862
Heo HW / 2008 / Construction of an Euchromatin Enriched Genomic DNA library in wheat and development of STS marker sets / Genome dynamics poster abstracts : 087 ~
Huang GQ / 2010 / Construction of a methylation filtration library in Hevea brasiliensis / Yi chuan 32 : 1071 ~ 1076
Bennetzen JL / 1994 / Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA / Genome 37 : 565 ~ 576
Gruenbaum Y / 1981 / Sequence specificity of methylation in higher plant DNA / Nature 292 : 860 ~ 862
Heo HW / 2008 / Construction of an Euchromatin Enriched Genomic DNA library in wheat and development of STS marker sets / Genome dynamics poster abstracts : 087 ~
Huang GQ / 2010 / Construction of a methylation filtration library in Hevea brasiliensis / Yi chuan 32 : 1071 ~ 1076
이 논문의 저자 정보
최근 본 자료
댓글(0)
0