지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
PCR-based assays with co-dominant or dominant allele-specific STS (sequence tagged sites) markers and sodiumdodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) have been used to discriminate Glu-1 and Glu-3 alleles in Korean wheat cultivars. Three alleles were identified at each high molecular weight glutenin (Glu-1) homoeologous locus and 10 alleles were identified at low molecular weight glutenin (Glu-3) homoeologous locus in Korean wheat cultivars. At Glu-1 loci,wheat cultivars contained Glu-A1a, b and c alleles amplified three fragments of 1093, 1063 and 920bp, and Glu-B1b, c and f alleles discriminated with 707bp (Glu-B1b and f) and 662bp (Glu-B1c) fragments and Glu-D1a, d and f alleles amplified two fragments576bp (Glu-D1d) and 612bp (Glu-D1a and f), respectively. At Glu-3 loci, wheat cultivars contained Glu-A3c, d and e alleles amplified a 573bp, 967bp and 158bp fragments, respectively, Glu-B3d, g, h and i alleles gave a 662bp, 853bp, 1022bp and 621bp, respectively and c) bands and Glu-D3a, b and c alleles amplified a 884bp (Glu-D3a and b) and 338bp (Glu-D3c)fragments. A multiplex-PCR assay was also established which permitted the discrimination of the major Glu-1 and Glu-3 to determine glutenin compositions in a single PCR reaction and agarose gel assay because these systems could be useful to select DNA-based identification of wheat lines carrying good bread making performance in Korean wheat breeding programs. Three multiplex-PCRs for Glu-1 and Glu-3, Glu-A1c/ Glu-B1acf/Glu-D1d, Glu-A3ac/Glu-A3d/Glu-A3e and Glu-B3d/Glu-B3fg /Glu-B3h were established in this study.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Branlard G / 2001 / Genetic diversity of wheat storage proteins and bread wheat quality / Euphytica 119 : 59 ~ 67
Branlard G / 2003 / Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.) / Genet. Res. Crop Evol 50 : 669 ~ 679
D’Ovidio R / 2004 / The low-molecular-weight glutenin subunits of wheat gluten / J. Cereal Sci 39 : 321 ~ 339
Eagles HA / 2002 / Estimation and utilization of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs / Aust. J. Agric. Research 53 : 367 ~
Flaete NES / 2003 / Association between allelic variation at the combined Gli-1, Glu-3 loci and protein quality in common wheat (Triticum aestivum L.) / J. Cereal Sci 37 : 12
Branlard G / 2003 / Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.) / Genet. Res. Crop Evol 50 : 669 ~ 679
D’Ovidio R / 2004 / The low-molecular-weight glutenin subunits of wheat gluten / J. Cereal Sci 39 : 321 ~ 339
Eagles HA / 2002 / Estimation and utilization of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs / Aust. J. Agric. Research 53 : 367 ~
Flaete NES / 2003 / Association between allelic variation at the combined Gli-1, Glu-3 loci and protein quality in common wheat (Triticum aestivum L.) / J. Cereal Sci 37 : 12
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