지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The objective of this study was to evaluate the suitability of simple sequence repeat markers for variety identification in 69 rose (Rosa x hybrida) varieties. A set of 112 SSR primer pairs was evaluated and 43 primer pairs showed polymorphism in 12 varieties. Twenty-two primer pairs out of 43 primer pairs showed high levels of polymorphism and reproducibility. The genetic relationship of 69 varieties was analyzed based on the marker genotypes of 22 SSRs. A total of 114 polymorphic amplified fragments were obtained by using 22 SSR markers. Two to ten SSR alleles were detected for each locus with an average of 5.18 alleles per locus. Average polymorphism information content (PIC) was 0.621, ranging from 0.211 to 0.813. A total of 114 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using unweighted pair-group method with arithmetical average (UPGMA). Cluster analysis of genetic diversity revealed that these SSR marker sets identified each genotypes of 69 rose varieties. These SSR markers may be used for wide range of practical application in variety identification of rose.
목차
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참고문헌 (21)
참고문헌 신청
Anderson JA / 1993 / Optimizing parental selection for genetic linkage maps / Genome 36 : 181 ~ 186
Bredemeijer GMM / 2002 / Construction and testing of a microsatellite database containing more than 500 tomato varieties / Theor Appl Genet 105 : 1019 ~ 1026
Esselink GD / 2003 / Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers / Theor Appl Genet 106 : 277 ~
Gi-Jun Kim / 2011 / Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars / 원예과학기술지 29 (3) : 240 ~ 246
Gudin S. / 2000 / Rose: genetics and breeding / Plant Breed Rev 17 : 159 ~ 189
Bredemeijer GMM / 2002 / Construction and testing of a microsatellite database containing more than 500 tomato varieties / Theor Appl Genet 105 : 1019 ~ 1026
Esselink GD / 2003 / Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers / Theor Appl Genet 106 : 277 ~
Gi-Jun Kim / 2011 / Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars / 원예과학기술지 29 (3) : 240 ~ 246
Gudin S. / 2000 / Rose: genetics and breeding / Plant Breed Rev 17 : 159 ~ 189
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