지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Tomato shelf-life is important for its fresh market usage especially for exporting and transporting tomato in tropical climate. To increase the shelf-life, delaying maturity is one of the powerful methods. A ripening gene related to the fruit maturity delaying was previously known through tomato mutation. RIPENING-INHIBITOR (Rin) from the recessive mutation effectively blocks the ripening process, and when the hybrid (Rin/rin) was formed it shows slow-ripening and long shelf-life. Since breeders need the long shelf-life character in the tomato breeding program, we had set out a series of experiments to develop a Rin related DNA marker. We searched for the proper primer sequences in the deletion region between Rin and rin mRNA sequences, and also in the intron region of rin. Primers that simultaneously amplified two bands with a co-dominant pattern were selected and converted to the Sequence Characterized Amplified Region (SCAR) marker. As a preliminary data, the SCAR marker clearly distinguished genotypes between heterozygote (Rin/rin) and homozygote (Rin/Rin, rin/rin), and this would help select the tomato lines that have a characteristic of long shelf-life.
목차
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참고문헌 (17)
참고문헌 신청
Adams-Phillips L / 2004 / Signal transduction systems regulating fruit ripening / Trends Plant Sci 9:331~338
Alvarez-Buyella ER / 2000 / MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes / Plant J 24:457~466
Barry CS / 2005 / Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato / Plant Physiol 138:267~275
Chang C / 1999 / The ethylene-response pathway: signal perception to gene regulation / Curr Opin Plant Biol 2:352~358
Giovannoni JJ / 1995 / Molecular genetic analysis of the ripeninginhibitor and non-ripening loci of tomato: a first step in genetic map-based cloning of fruit ripening genes / Mol Gen Genet 248:195~206
Alvarez-Buyella ER / 2000 / MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes / Plant J 24:457~466
Barry CS / 2005 / Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato / Plant Physiol 138:267~275
Chang C / 1999 / The ethylene-response pathway: signal perception to gene regulation / Curr Opin Plant Biol 2:352~358
Giovannoni JJ / 1995 / Molecular genetic analysis of the ripeninginhibitor and non-ripening loci of tomato: a first step in genetic map-based cloning of fruit ripening genes / Mol Gen Genet 248:195~206
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