지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The study was carried out to evaluate growth and genetic diversity among in vitro cultured protocorms of Cymbidium spp. that irradiated with 10 Gy～300 Gy of electron beam. Shoot induction and shoot length were decreased by an increase of intensity of electron beam, and shoot induction was significantly decreased over 200 Gy of electron beam irradiation in 10 weeks culture. As a result of ISSR (inter-simple sequence repeats) analysis, out of 80 bands detected overall, 10 bands were identified to be polymorphic with a rate of 12.5% at control group, and 90 bands detected overall, 38 bands were identified to be polymorphic with a rate of 42.2% at electron beam treatment. According to the RAPD (random amplified polymorphic DNA)analysis, out of 92 bands detected overall, 12 bands were identified to be polymorphic with a rate of 13.0% at control group,and 121 bands detected overall, 53 bands were identified to be polymorphic with a rate of 43.8% at electron beam treatment.
Genetic similarity matrix (GSM) of electron beam treatment was lower than that of control. The result indicates that the beam treatment accelerated genetic diversity by promoting polymorphism of DNA. According to the cluster analysis by using both ISSR and RAPD markers, individuals of C. spp. were divided into control and electron beam treatment group. Our results indicate that the electron beam irradiated in vitro cultured C. spp. might be resulted in promoting higher polymorphism but decreasing GSM.
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참고문헌 (27)
참고문헌 신청
[학술지
Abe T / 2000 / Stress-tolerant mutants induced by heavy-ion beams / Proceedings of 39th Gamma Field Symposium 39 : 45 ~ 56
Bae CH / 1999 / Characterization of in vitro growth and differentiation of an albino mutant of Nicotiana tobacum L / Plant Biotechnol 26 (3) : 197 ~ 203
Bae CH / 2000 / Characterization of a periclinal chimera variegated tobacco (Nicotiana tabacum L) / Plant Sci 151 : 93 ~ 101
Bae CH / 2000 / Effect of a heavy-ion beam irradiation on anthers, calli and seeds of tobacco (Nicotiana tabacum L. cv. BY-4 / Plant Biotechnol 27 (2) : 109 ~ 115
Abe T / 2000 / Stress-tolerant mutants induced by heavy-ion beams / Proceedings of 39th Gamma Field Symposium 39 : 45 ~ 56
Bae CH / 1999 / Characterization of in vitro growth and differentiation of an albino mutant of Nicotiana tobacum L / Plant Biotechnol 26 (3) : 197 ~ 203
Bae CH / 2000 / Characterization of a periclinal chimera variegated tobacco (Nicotiana tabacum L) / Plant Sci 151 : 93 ~ 101
Bae CH / 2000 / Effect of a heavy-ion beam irradiation on anthers, calli and seeds of tobacco (Nicotiana tabacum L. cv. BY-4 / Plant Biotechnol 27 (2) : 109 ~ 115
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