지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The main objective of this study was to develop the multi-resistance lines to insects(brown planthopper; BPH, rice green leafhopper; GRH) and disease(blast; BL, bacterial blight; BB and rice stripe virus disease;RSV) with good grain quality and plant type by combining conventional breeding and marker assisted selection(MAS) and to eliminate the linkage drag effects between Bph1 gene and culm length, we conducted MAS of Bph1 gene in advanced backcross and double cross progenies.
‘Nampyeong’, ‘Junam’ and ‘Milyang220’ were used as the parent in this study. ‘Milyang220’ was used as the donor of brown planthopper resistance gene Bph1 with tall culm length. Two backcross progenies were developed using two recipients ‘Nampyeong’ carrying GRH resistance gene Grh3(t) with good grain appearance and ‘Junam’ harboring bacterial blight resistance gene Xa3 with short culm length. Two BC1 generations were resulted from the backcrossing of the F1 plants with recurrent parents ‘Nampyeong’ and ‘Junam’. The second rounds of backcrossing(BC2) were derived from the cross of selected resistant BC1F1 plants based on heterozygous genotype of RM28493 linked to Bph1 gene. The double crossed population was constructed from the cross of between each heterozygous BC2F1 plants at RM28493 locus of ‘Nampyeong*3 / Milyang220’ and ‘Junam*3/ Milyang220’., The homozygous alleles in Bph1 gene were selected using co-dominant DNA marker RM28493 in double crossed population. Eighty-five lines with multi-resistance to BL, BB, RSV, GRH and BPH were selected by bio-assay and MAS in generation of double crossing. The culm length, head rice ratio and yield of the selected multi resistance lines was ranged from 71 to 88 cm, from 51 to 93%, from 449 to 629 kg/10a. respectively. We can select a promising multi resistance line similar with ‘Nampyeong’ of major agronomic traits such as culm legnth, head rice ratio and yield. It was designated as Milyang265.
Finally this study was developed the multi resistant varieties against to insects and diseases with the good grain quality ‘Milyang265’ by the advanced backcross and double cross combining MAS and it can be used as genetic resources of multi-resistance to insect and diseases in rice breeding programs.
목차
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참고문헌 (25)
참고문헌 신청
Bonman JM / 1992 / Breeding Rice for Resistance to Pests / Annu. Rev. in Phytopathol 30 : 507 ~ 528
Cuong HV / 2009 / Rice dwarf disease in North Vietnam in 2009 is caused by southern rice black-streaked dwarf virus (SRBSDV). / Bull. Inst. Trop. Agr 32 : 85 ~ 92
Hayano-Saito Y / 2000 / SCAR marker selection of the rice stripe resistance gene Stvb-i / Breeding Research 2 : 67 ~ 70
Jairin J / 2009 / Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection / Fie
Jong-Hee Lee / 2007 / Identification of DNA Marker for Brown Planthopper Resistance gene Bph1 in Rice (Oryza sativa L.) / 한국육종학회지 39 (2) : 172 ~ 177
Cuong HV / 2009 / Rice dwarf disease in North Vietnam in 2009 is caused by southern rice black-streaked dwarf virus (SRBSDV). / Bull. Inst. Trop. Agr 32 : 85 ~ 92
Hayano-Saito Y / 2000 / SCAR marker selection of the rice stripe resistance gene Stvb-i / Breeding Research 2 : 67 ~ 70
Jairin J / 2009 / Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection / Fie
Jong-Hee Lee / 2007 / Identification of DNA Marker for Brown Planthopper Resistance gene Bph1 in Rice (Oryza sativa L.) / 한국육종학회지 39 (2) : 172 ~ 177
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