지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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This study was conducted to investigate the genetic diversity and to develop a technique for cultivar identification using SSR markers in grapevine. Thirty Korean bred and introduced grapevine cultivars were evaluated by 28 SSR markers. A total of 143 alleles were produced ranging from 2 to 8 alleles with an average of 5.1 alleles per locus. Polymorphic information contents (PIC) were ranged from 0.666 (VVIp02) to 0.975 (VVIn33 and VVIn62) with an average of 0.882. UPGMA (unweighted pair-group method arithmetic average) clustering analysis based on genetic distances using 143 alleles classified 30 grapevine cultivars into 7 clusters by similarity index of 0.685. Similarity values among the tested grapevine cultivars ranged from 0.575to 1.00, and the average similarity value was 0.661. The similarity index was the highest (1.00) between ‘Jinok’ and ‘Campbell Early’, and the lowest (0.575) between ‘Alden’ and ‘Narsha’. The genetic relationships among the 30 studied grapevine cultivars were basically consistent with the known pedigree. The three SSR markers sets (VVIn61, VVIt60, and VVIu20) selected from 28 primers were differentiated all grapevine cultivars except for ‘Jinok’ and ‘Campbell Early’. Five cultivars (‘Narsha, ‘Alden’,‘Dutchess’, ‘Pione’, and ‘Muscat Hamburg’) were identified by VVIn61 at the first step. Then 21 cultivars including ‘Hongsodam’by VVIt60 at the second step and 2 cultivars (‘Heukbosuck’ and ‘Suok’) by VVIu20 at the third step were identified. These markers could be used as a reliable tool for the identification of Korean grapevine cultivars.
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참고문헌 (25)
참고문헌 신청
Almadanim MC / 2007 / Genetic diversity of the grapevine (Vitis vinifera L.) cultivars most utilized for wine production in Portugal / Vitis 46 : 119 ~ 119
Aranzana MJ / 2002 / Development and variability analysis of microsatellite markers in peach / Plant Breed 121 : 87 ~ 92
Arias RS / 2009 / UPIC: Pearl scripts to determine the number of SSR markers to run / Bioinformation 3 : 352 ~ 360
Baneh HD / 2009 / Analysis of SSR and AFLP markers to detect genetic diversity among selected clones of grapevine (Vitis vinifera L.) cv. Keshmeshi / S Afr J Enol Vitic 30 :
Bowers JE / 1996 / Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.) / Genome 39 : 628 ~ 633
Aranzana MJ / 2002 / Development and variability analysis of microsatellite markers in peach / Plant Breed 121 : 87 ~ 92
Arias RS / 2009 / UPIC: Pearl scripts to determine the number of SSR markers to run / Bioinformation 3 : 352 ~ 360
Baneh HD / 2009 / Analysis of SSR and AFLP markers to detect genetic diversity among selected clones of grapevine (Vitis vinifera L.) cv. Keshmeshi / S Afr J Enol Vitic 30 :
Bowers JE / 1996 / Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.) / Genome 39 : 628 ~ 633
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