지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Conventional staining and fluorescence in situ hybridization (FISH) karyotypes of the non-genetically modified (GM)parental rice line, ‘Nakdong’ (Oryza sativa L. japonica), and its four GM rice lines, LS28 (event LS30-32-20-1), Cry1Ac1 (event C7-1-9-1), and LS28 × Cry1Ac1 (events L/C1-1-3-1 and L/C1-3-1-1) were analyzed using 5S and 45S rDNAs as probes. Both parental and transgenic lines were diploids (2n=24) with one satellite chromosome pair. The lengths of the prometaphase chromosomes ranged from 1.50 to 6.30 μm. Four submetacentric and eight metacentric pairs comprised the karyotype of ‘Nakdong’ and its four GM lines. One pair of 5S rDNA signals was detected near the centromeric region of chromosome g in both the parental and transgenic lines. The 45S rDNA signals were detected on the secondary constrictions of the satellite chromosome pair in both the parental and transgenic lines. There was no significant difference in chromosome size, length, and composition between ‘Nakdong’ and its four GM lines. This research was conducted as a preliminary study for chromosomal detection of transgenes in GM rice lines and would be useful for their breeding programs.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Ansari A / 1999 / Persistence of an alternate chromatin structure at silenced loci in vitro / Proc. Natl. Acad. Sci 96 : 343 ~ 348
Arumuganathan K / 1991 / Nuclear DNA content of some important plant species / Plant Mol. Biol. Rep 9 : 208 ~ 218
Baum B / 2001 / The 5S rDNA gene sequence variation in wheats and some polyploidy wheat progenitors (Poaceae: Triticeae) / Gen. Res. Crop Evol 48 : 35 ~ 51
Buell CR / 2005 / Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species / Genome Res 15 : 1284 ~ 1291
Cheng Z / 2001 / Toward a cytological characterization of the rice genome / Genome Res 11 : 2133 ~ 2141
Arumuganathan K / 1991 / Nuclear DNA content of some important plant species / Plant Mol. Biol. Rep 9 : 208 ~ 218
Baum B / 2001 / The 5S rDNA gene sequence variation in wheats and some polyploidy wheat progenitors (Poaceae: Triticeae) / Gen. Res. Crop Evol 48 : 35 ~ 51
Buell CR / 2005 / Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species / Genome Res 15 : 1284 ~ 1291
Cheng Z / 2001 / Toward a cytological characterization of the rice genome / Genome Res 11 : 2133 ~ 2141
이 논문의 저자 정보
최근 본 자료
댓글(0)
0