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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Three low molecular weight (LMW) glutenin subunit genes were isolated from hexaploid wheat cultivars (Triticum aestivum. L) using LMW-GS specific primers and designated as Jokyung II-2, CS III-5 and Keumkang 6-12, respectively. All sequences were classified as LMW-i type genes based on the presence of an N-terminal isoleucine residue. All comprised of four regions, the signal peptide, N- and C- terminal domains, and a repetitive domain. Although they showed high similarity with other LMW-i type genes from hexaploid bread wheat, they also displayed unique features. Particularly, Keumkang 6-12 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the A–G transition, namely tyrosine-cysteine substitution at position 335 from the N-terminal end. In addition, a total of 15 SNPs and one insertions/deletions (InDels) were detected among Jokyung II-2, CS III-5 and Jokyung HQ6199333 and 10 other LMW-i genes. Two dimensional gel electrophoresis and LC-ESI MS/MS analysis revealed that LMW i-type glutenin subunit of the Jokyung is Glu-A3c. The phylogenic analysis showed that LMW i-type genes were divided roughly into diploid and hexaploid groups and the hexaploid were further classified into 7 subgroups (Glu-A3a~g). We also found length polymorphism in the repetitive domain showing 2~179 amino acid residues variation among the previously published LMW-i type glutenin subunits. The length polymorphism can be used as reliable genetic marker for use in marker-assisted breeding to select for specific alleles of the quality-determining locus in wheat breeding program.
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참고문헌 (22)
참고문헌 신청
An X / 2006 / Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.) / Theor Appl Genet 113 : 383
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Branlard G / 2001 / Genetic diversity of wheat storage proteins and bread wheat quality / Euphytica 119 : 59 ~ 67
Cloutier S / 2001 / Cloning and expression of a LMW-i glutenin gene / J. Cereal Sci 33 : 143 ~ 154
Colot V / 1989 / Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes / Mol Gen Genet 216 : 81 ~ 90
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Branlard G / 2001 / Genetic diversity of wheat storage proteins and bread wheat quality / Euphytica 119 : 59 ~ 67
Cloutier S / 2001 / Cloning and expression of a LMW-i glutenin gene / J. Cereal Sci 33 : 143 ~ 154
Colot V / 1989 / Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes / Mol Gen Genet 216 : 81 ~ 90
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