지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Totally, 26 collections, 17 from Korea and 9 from China, were investigated for their sequences of 5S rDNA, especially the non-transcribed spacers (NTSs). Sequences of 5S rDNA were isolated by PCR using the primers, 5s-rRNA1 and 5s-rRNA2.
Genomic DNA PCR produced single amplification of 300, 330, or 350 base pair fragments. Sequence analysis revealed that all inserts contained the part of 5S rDNA gene sequence and the full length of the NTS region. Three different sizes of the fragments were confirmed due to different size of NTS and their length were 300bp, 330bp and 350bp, respectively. Among 17 Korean foxtail millets tested, 14 collections showed single 300bp amplification. Longest fragment amplification, 350bp, was obtained only from the foxtail millet from China origin, even though 2 of them include 300bp fragment. CLUSTALW multiple alignments of 26 foxtail millets clearly revealed 4 areas with certain degree of sequence heterogeneity (region I, II, III, IV). Among 4 boxed areas, foxtail millet genotypes from China have distinct insertion especially in region III. Five of them have extra insertion of sequence and their additional sequences were either 45 or 48 base pair. Three Korean foxtail millets have 32 bp insertion. Other 8 Korean collections have short insert sequences (6 to 8 bp), 3 with 8 bp and 5 with 6 bp. In addition to insert, deletion sequences were also confirmed as major deletion was observed in region II of Chinese collection. The size of deletion was 7bp long. According to phylogenic tree constructed using MEGA4 program, clear grouping was not revealed. To obtain more convincing results various collections from many countries should be obtained and analyzed to distinguish different germplasm from different origin.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Baum BR / 1994 / The molecular diversity of the 5S rRNA gene in barley (Hordeum vulgare) / Genome 37 : 992 ~ 998
Buckler IV ES / 1996 / Zea systematics: ribosomal ITS evidence / Mol. Biol. Evol 13 : 612 ~ 622
Falistocco E / 2007 / Investigations of 5S rDNA of Vitis vinifera L.: sequence analysis and physical mapping / Genome 50 (10) : 927 ~ 938
Fukunaga K / 1997 / Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv. and a survey of variation from Europe and Asia / Theor. Appl. Genet 97 : 751 ~ 7
Gupta S / 2012 / Sequence-based novel genomic microsatellite markers for robust genotyping purposes in foxtail millet [Setaria italica (L.) P. Beauv] / Plant Cell Rep 31 (2)
Buckler IV ES / 1996 / Zea systematics: ribosomal ITS evidence / Mol. Biol. Evol 13 : 612 ~ 622
Falistocco E / 2007 / Investigations of 5S rDNA of Vitis vinifera L.: sequence analysis and physical mapping / Genome 50 (10) : 927 ~ 938
Fukunaga K / 1997 / Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv. and a survey of variation from Europe and Asia / Theor. Appl. Genet 97 : 751 ~ 7
Gupta S / 2012 / Sequence-based novel genomic microsatellite markers for robust genotyping purposes in foxtail millet [Setaria italica (L.) P. Beauv] / Plant Cell Rep 31 (2)
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