지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
EST-SSRs were developed in Brassica napus by database mining. We isolated 7,802 EST-SSRs from the B. napus 643,946 ESTs deposited in the NCBI. With the cut-off value of >10 repeats in di-nucleotide repeats and >7 tri-nucleotide repeats,303 ESTs were suitable for primer designing for PCR amplification. Of the sixteen possible di-nucleotide combinations, only three types of repeats (AC/GT, AG/CT, and AT/TA) were present among the di-nucleotide EST-SSRs. Whereas, 27 tri-nucleotide repeat motifs from the 64 possible combinations were present. The repeat numbers ranged from 10-15 in di-nucleotide repeats and 7-9 in tri-nucleotide repeat motifs, respectively. By checking PCR amplification in 10 Korean rapeseed breeding lines or cultivars, 234 primer pairs showed successful PCR amplification and 142 of the 234 primer pairs revealed polymorphism among the control cultivars or breeding lines. While the repeat length does not related with the SSR polymorphisms, the repeat motif number showed positive relation with the polymorphism generation by showing higher repeat numbers with higher polymorphisms. We are here presenting the PCR amplifiable primer sequences of the 234 SSR primer pairs to aid in the germplasm management and breeding programs of the B. napus in Korea.
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참고문헌 (24)
참고문헌 신청
Aggarwal RK / 2007 / Idenfication, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species / Theor. App
Batley J / 2007 / Identification and characterization of simple sequence repeat markers from Brassica napus expressed sequences / Mol. Ecol. Notes 7 : 886 ~ 889
Cheng X / 2009 / Development and mapping of microsatellite markers from genome survey sequences in Brassica napus / Theor. Appl. Genet 118 : 1121 ~ 1131
Dhandapani V / 2012 / Development of EST database and transcriptome analysis in the leaves of Brassica rapa using a newly developed pipeline / Genes Genom 34
Gao C / 2011 / Characterization and comparison of gene-based simple sequence repeats across Brassica species / Mol. Genet. Genomics 286 : 161 ~ 170
Batley J / 2007 / Identification and characterization of simple sequence repeat markers from Brassica napus expressed sequences / Mol. Ecol. Notes 7 : 886 ~ 889
Cheng X / 2009 / Development and mapping of microsatellite markers from genome survey sequences in Brassica napus / Theor. Appl. Genet 118 : 1121 ~ 1131
Dhandapani V / 2012 / Development of EST database and transcriptome analysis in the leaves of Brassica rapa using a newly developed pipeline / Genes Genom 34
Gao C / 2011 / Characterization and comparison of gene-based simple sequence repeats across Brassica species / Mol. Genet. Genomics 286 : 161 ~ 170
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