지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Brachypodium distachyon is rapidly emerged in biological study and has been currently used as a model system for genetics and functional studies for crop improvement and biofuel production. Phosphinothricin (PPT) has been widely used as a selectable agent, which raises ammonium content and induces toxicity in non-transformed plant cells. However PPT selection is not much effective on Brachypodium callus consequently reducing transformation efficiency. In order to identify the efficient conditions of PPT selection, calli obtained from mature seeds of Brachypodium (PI 254867) were cultured on the callus inducing medium (CIM) or regeneration medium (ReM) containing serial dilutions of the PPT (0, 2, 5, 10, and 15 mg/l) in dark or light condition. Callus growth and ammonium content of each treatment were measured 2 weeks after the treatment. Although callus growth and ammonium content did not show much difference in CIM, slow callus growth and increased ammonium accumulation were found in ReM. No significant difference of ammonium accumulation in response to PPT was found between dark and light conditions. In order to identify major factors affecting increased ammonium accumulation, callus was cultured on the media in combined with phytohormones (2,4-D or kinetin) and carbon sources (sucrose or maltose) containing with PPT (5 mg/l). The highest ammonium content in callus was found in the kinetin and maltose media.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Alves SC / 2009 / A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21 / Nat. Protoc. 4 : 638 ~ 649
Bablak P / 1995 / Plant regeneration and micropropagation of Brachypodium distachyon / Plant Cell Tiss. Org. 42 : 97 ~ 107
Christiansen P / 2005 / A rapid and efficient transformation protocol for the grass Brachypodium distachyon / Plant Cell Rep. 23 : 751 ~ 758
De Block M / 1987 / Engineering herbicide resistance in plants by expression of a detoxifying enzyme / EMBO J. 6 (9) : 2513 ~ 2518
De Block M / 1995 / The selection mechanism of phosphinothricin is influenced by the metabolic status of the tissue / Planta. 197 : 619 ~ 626
Bablak P / 1995 / Plant regeneration and micropropagation of Brachypodium distachyon / Plant Cell Tiss. Org. 42 : 97 ~ 107
Christiansen P / 2005 / A rapid and efficient transformation protocol for the grass Brachypodium distachyon / Plant Cell Rep. 23 : 751 ~ 758
De Block M / 1987 / Engineering herbicide resistance in plants by expression of a detoxifying enzyme / EMBO J. 6 (9) : 2513 ~ 2518
De Block M / 1995 / The selection mechanism of phosphinothricin is influenced by the metabolic status of the tissue / Planta. 197 : 619 ~ 626
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