지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In this work, we analyzed the activity of control enzymes of flower color biosynthesis, chalcone synthase (CHS) and
flavanone 3-hydroxylase (FHT) using biochemical and enzymological methods in Lilium longiflorum and 11 Lilium cultivars. The
results obtained are as follows ; Naringenin (NAR) was synthesized in all Lilium cultivars tested by the catalytic activity of CHS
which used malonyl-CoA and 4-coumaryol-CoA as substrates. Substrate-specific activity of CHS was observed because eridictiol
(ERI), which uses caffeoyl-CoA as a substrate, was not detected in tested cultivars. In next step, dihydroflavone product was
synthesized by FHT using flavanones as a substrate. FHT synthesized dihydrokaempferol (DHK) by using NAR as substrates.
A remarkable activity of FHT was observed in other 11 cultivars.
목차
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참고문헌 (25)
참고문헌 신청
Britsch L / 1986 / Purification and characterization of (2S)-flavanone 3-hydroxylase from Petunia hybrida / Eur. J. Biochem 156 : 569 ~ 577
Britsch L / 1992 / Molecular cloning, sequence analysis and in vitro expression of flavanone 3-hydroxylase from Petunia hybrida / J. Biol. Chem 267 : 5380 ~ 5387
Britsch L / 1993 / Molecular characterization of flavanone 3-hydroxylases. consensus sequence, comparison with related enzymes and the role of conserved residues / Eur. J. Bi
Britsch l / 1990 / Purification and characterization of flavone synthase Ⅰ, a 2-oxoglutarate-dependent desaturase / Archives of Biochemistry and Biophysics 276 : 348 ~ 354
Choi GT / 2002 / Genetic modulation of flower color intensity by newly developed dominant-negative CHS. J. Plant. Biotechnol / Proceedings of the 9th Symposium : 69 ~ 72
Britsch L / 1992 / Molecular cloning, sequence analysis and in vitro expression of flavanone 3-hydroxylase from Petunia hybrida / J. Biol. Chem 267 : 5380 ~ 5387
Britsch L / 1993 / Molecular characterization of flavanone 3-hydroxylases. consensus sequence, comparison with related enzymes and the role of conserved residues / Eur. J. Bi
Britsch l / 1990 / Purification and characterization of flavone synthase Ⅰ, a 2-oxoglutarate-dependent desaturase / Archives of Biochemistry and Biophysics 276 : 348 ~ 354
Choi GT / 2002 / Genetic modulation of flower color intensity by newly developed dominant-negative CHS. J. Plant. Biotechnol / Proceedings of the 9th Symposium : 69 ~ 72
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